Cross-Reactive Immunity to Mycobacterium tuberculosis DosR Regulon-Encoded Antigens in Individuals Infected with Environmental, Nontuberculous Mycobacteria
| Autor: | Annemieke H. Friggen, T. B. K. Reddy, S. M. Arend, Michèl R. Klein, Marleen J. C. Verduyn, Tom H. M. Ottenhoff, Krista E. van Meijgaarden, Kees L. M. C. Franken, Gary K. Schoolnik, May Young Lin |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Tuberculosis Immunology Enzyme-Linked Immunosorbent Assay Cross immunity Cross Reactions Regulon Microbiology Mycobacterium Mycobacterium tuberculosis Interferon-gamma Bacterial Proteins Immunity medicine Humans Antigens Bacterial Mycobacterium Infections biology Latent tuberculosis Tuberculin Test Middle Aged biology.organism_classification medicine.disease Virology DNA-Binding Proteins Infectious Diseases Microbial Immunity and Vaccines Female Parasitology Nontuberculous mycobacteria Bacterial antigen Protein Kinases |
| Zdroj: | Infection and Immunity. 77:5071-5079 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.00457-09 |
| Popis: | Mycobacterium tuberculosis DosR regulon-encoded antigens are highly immunogenic in M. tuberculosis -infected humans and are associated with latent tuberculosis infection. We have investigated the hypothesis that infection with or exposure to nontuberculous mycobacteria (NTM) can induce cross-reactive immunity to M. tuberculosis DosR regulon-encoded antigens since responsiveness has been observed in non- M. tuberculosis -exposed but purified protein derivative-responsive individuals. M. tuberculosis DosR regulon-encoded antigen-specific T-cell responses were studied in peripheral blood mononuclear cells (PBMCs) of NTM-infected/exposed individuals. BLASTP was used to determine the presence of M. tuberculosis DosR regulon-encoded protein orthologs among environmental mycobacteria and nonmycobacteria. Significant gamma interferon production was observed in PBMCs from NTM-infected/exposed individuals in response to M. tuberculosis DosR regulon-encoded antigens. DosR regulon-encoded protein orthologs were prominently present in tuberculous and environmental mycobacteria and surprisingly also in nonmycobacteria. The ubiquitous presence of the highly conserved DosR master regulator protein Rv3133c suggests that this is a general adaptive bacterial response regulator. We report a first series of M. tuberculosis antigens to which cross-reactive immunity is induced by NTM infection/exposure. The high conservation of M. tuberculosis DosR regulon-encoded antigens most likely enables them to induce cross-reactive T-cell responses. |
| Databáze: | OpenAIRE |
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