Multiplex PCR methods for detection of several viruses associated with canine respiratory and enteric diseases
| Autor: | Yuwei He, Xiangqi Hao, Shoujun Li, Pei Zhou, Weiqi Xiao, Qingxu Zheng, Pan Tao, Xiangyu Xiao, Ruohan Liu, Xi Lin |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
RNA viruses
0301 basic medicine Pulmonology Coronaviruses viruses Canine influenza Artificial Gene Amplification and Extension Pathology and Laboratory Medicine medicine.disease_cause Polymerase Chain Reaction 0403 veterinary science Coronavirus Canine Medicine and Health Sciences Dog Diseases Enterovirus Coronavirus Mammals Multidisciplinary biology Canine parvovirus Eukaryota Canine coronavirus General Medicine 04 agricultural and veterinary sciences Infectious Diseases Medical Microbiology Viral Pathogens Vertebrates Viruses Coinfection Medicine Pathogens General Agricultural and Biological Sciences Coronavirus Infections Research Article 040301 veterinary sciences Science Research and Analysis Methods Microbiology Canine Coronavirus General Biochemistry Genetics and Molecular Biology Virus 03 medical and health sciences Dogs Canine Distemper Virus Multiplex polymerase chain reaction Enterovirus Infections medicine Animals Molecular Biology Techniques Molecular Biology Microbial Pathogens Canine distemper business.industry Organisms Biology and Life Sciences Respiration Disorders biology.organism_classification medicine.disease Virology 030104 developmental biology Co-Infections Respiratory Infections Amniotes Paramyxoviruses business Multiplex Polymerase Chain Reaction |
| Zdroj: | PLoS ONE, Vol 14, Iss 3, p e0213295 (2019) PLoS ONE PLOS ONE |
| ISSN: | 1932-6203 |
| Popis: | Viral respiratory and intestinal infections are the most common causes of canine viral illness. Infection with multiple pathogens occurs in many cases. Rapid diagnosis of these multiple infections is important for providing timely and effective treatment. To improve diagnosis, in this study, two new multiplex polymerase chain reactions (mPCRs) were developed for simultaneous detection of canine respiratory viruses (CRV) and canine enteric viruses (CEV) using two separate primer mixes. The viruses included canine adenovirus type 2 (CAV-2), canine distemper virus (CDV), canine influenza virus (CIV), canine parainfluenza virus (CPIV), canine circovirus (CanineCV), canine coronavirus (CCoV) and canine parvovirus (CPV). The sensitivity of the mPCR results showed that the detection limit of both mPCR methods was 1×104 viral copies. Twenty nasal swabs (NS) and 20 anal swabs (AS) collected from dogs with symptoms of respiratory disease or enteric disease were evaluated using the novel mPCR methods as a clinical test. The mPCR protocols, when applied to these respiratory specimens and intestinal samples, could detect 7 viruses simultaneously, allowing rapid investigation of CRV (CAV-2, CDV, CIV and CPIV) and CEV (CAV-2, CanineCV, CCoV and CPV) status and prompt evaluation of coinfection. Our study provides an effective and accurate tool for rapid differential diagnosis and epidemiological surveillance in dogs. |
| Databáze: | OpenAIRE |
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