Modification of the Peptidoglycan of Escherichia coli in the Viable But Nonculturable State
| Autor: | Maria M. Lleo, Pietro Canepari, Caterina Signoretto |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Glycan
Penicillin binding proteins Peptidoglycan Muramoylpentapeptide Carboxypeptidase medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Viable but nonculturable chemistry.chemical_compound Bacteriolysis Bacterial Proteins Escherichia coli medicine Penicillin-Binding Proteins Muramidase Chromatography High Pressure Liquid chemistry.chemical_classification Antigens Bacterial biology General Medicine biology.organism_classification Enzyme Hexosyltransferases chemistry Biochemistry Peptidyl Transferases biology.protein Carrier Proteins Bacteria |
| Zdroj: | Current Microbiology. 44:125-131 |
| ISSN: | 1432-0991 0343-8651 |
| DOI: | 10.1007/s00284-001-0062-0 |
| Popis: | The aim of this study was to analyse the chemical composition of peptidoglycan and the state of some of the enzymes involved in its metabolism in Escherichia coli KN126 in the viable but nonculturable (VBNC) state which is a survival strategy adopted by bacteria (including those of medical interest) when exposed to environmental stresses. When entering the VBNC state, E. coli cells miniaturised and became coccus-shaped. Analysis of peptidoglycan chemical composition, by separation in HPLC of muropeptides released by muramidase digestion of purified peptidoglycan, indicated a high degree of cross-linking, a threefold increase in unusual DAP-DAP cross-linking, an increase in muropeptides bearing covalently bound lipoprotein, and a shortening of the average length of glycan strands in comparison with dividing cells. Analysis of penicillin-binding proteins (PBPs), enzymes involved in the terminal stage of peptidoglycan assembly showed the disappearance of high-molecular-weight PBPs 1A, 1B, 2, and 3 in VBNC cells. Finally, VBNC cells displayed an autolytic capability which was far higher than that of exponentially growing cells. It is suggested that part of these alterations of peptidoglycan may be connected with the VBNC state. |
| Databáze: | OpenAIRE |
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