Antiparasitic efficacy of dihydrosanguinarine and dihydrochelerythrine from Macleaya microcarpa against Ichthyophthirius multifiliis in richadsin (Squaliobarbus curriculus)
| Autor: | Xu Yang, Shen Jinyu, Hao Guijie, Yao Jiayun, Yin Wenlin, Pan Xiaoyi, Hong-shun Ru, Zhi-Ming Zhou, Xi-Lian Li |
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| Rok vydání: | 2011 |
| Předmět: |
Gills
Magnetic Resonance Spectroscopy Time Factors Antiparasitic medicine.drug_class Antiprotozoal Agents Cyprinidae Squaliobarbus curriculus Ciliophora Infections Biology Median lethal dose Mass Spectrometry Microbiology Lethal Dose 50 Fish Diseases Column chromatography Parasitic Sensitivity Tests Papaveraceae medicine Confidence Intervals Bioassay Animals Hymenostomatida Benzophenanthridines General Veterinary Ichthyophthirius multifiliis Traditional medicine Plant Extracts General Medicine biology.organism_classification Isoquinolines Macleaya microcarpa Plant Leaves Parasitology Biological Assay |
| Zdroj: | Veterinary parasitology. 183(1-2) |
| ISSN: | 1873-2550 |
| Popis: | Ichthyophthirius multifiliis is a holotrichous protozoan that invades the gills and skin surfaces of fish and can cause morbidity and high mortality in most species of freshwater fish worldwide. The present study was undertaken to investigate the antiparasitic activity of crude extracts and pure compounds from the leaves of Macleaya microcarpa. The chloroform extract showed a promising antiparasitic activity against I. multifiliis. Based on these finding, the chloroform extract was fractionated on silica gel column chromatography in a bioactivity-guided isolation affording two compounds showing potent activity. The structures of the two compounds were elucidated as dihydrosanguinarine and dihydrochelerythrine by hydrogen and carbon-13 nuclear magnetic resonance spectrum and electron ionization mass spectrometry. The in vivo tests revealed that dihydrosanguinarine and dihydrochelerythrine were effective against I. multifiliis with median effective concentration (EC(50)) values of 5.18 and 9.43 mg/l, respectively. The acute toxicities (LC(50)) of dihydrosanguinarine and dihydrochelerythrine for richadsin were 13.3 and 18.2mg/l, respectively. The overall results provided important information for the potential application of dihydrosanguinarine and dihydrochelerythrine in the therapy of serious infection caused by I. multifiliis. |
| Databáze: | OpenAIRE |
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