Extracellular vesicles from follicular fluid may improve the nuclear maturation rate of in vitro matured mare oocytes

Autor:	Julia Gabryś, Barbara Kij-Mitka, Sebastian Sawicki, Joanna Kochan, Agnieszka Nowak, Julianna Łojko, Elżbieta Karnas, Monika Bugno-Poniewierska
Rok vydání:	2022
Předmět:	Cumulus Cells Equine COC in vitro maturation follicular fluid Follicular Fluid In Vitro Oocyte Maturation Techniques Extracellular Vesicles Ovarian Follicle Food Animals mare Oocytes oocytes Animals Female Animal Science and Zoology Horses extracellular vesicles Small Animals
Zdroj:	Theriogenology. 188:116-124
ISSN:	0093-691X
DOI:	10.1016/j.theriogenology.2022.05.022
Popis:	The in vitro maturation (IVM) of equine oocytes is still not efficient and does not yield consistent results. The specific requirements of equine oocytes during this process are still largely unknown, which hinders the development of assisted reproductive techniques (ART) in this species. Because the ovarian follicle microenvironment supports oocytes in their acquisition of developmental competence, follicular fluid seems to be a substantial source of bioactive factors that could support the IVM process. Extracellular vesicles (EVs) are cell-secreted molecules in body fluids that are able to deliver molecular signals and transfer genetic information (mRNA, miRNA) between donor and recipient cells. Hence, our hypothesis is that follicular fluid EVs (ffEVs) from small (
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae878975638a1796f9232d6e0d46901c https://doi.org/10.1016/j.theriogenology.2022.05.022 Zobrazit plný text záznamu Full Text from ScienceDirect