Single nuclei RNA-seq of mouse placental labyrinth development
| Autor: | Robert Blelloch, Bryan Marsh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cell type
placenta QH301-705.5 Science Cell RNA-Seq Biology General Biochemistry Genetics and Molecular Biology Mice Pregnancy Placenta medicine Animals Biology (General) mouse syncytiotrophoblasts General Immunology and Microbiology single nuclei RNA sequencing Sequence Analysis RNA General Neuroscience Embryogenesis placental development RNA Cell Differentiation General Medicine Cell biology labyrinth Mice Inbred C57BL medicine.anatomical_structure Gene Expression Regulation embryonic structures Medicine Female Chorionic Villi Single-Cell Analysis Transcriptome Developmental biology Function (biology) Research Article Developmental Biology |
| Zdroj: | eLife, Vol 9 (2020) eLife |
| Popis: | The placenta is the interface between mother and fetus in all eutherian species. However, our understanding of this essential organ remains incomplete. A substantial challenge has been the syncytial cells of the placenta, which have made dissociation and independent evaluation of the different cell types of this organ difficult. Here, we address questions concerning the ontogeny, specification, and function of the cell types of a representative hemochorial placenta by performing single nuclei RNA sequencing (snRNA-seq) at multiple stages of mouse embryonic development focusing on the exchange interface, the labyrinth. Timepoints extended from progenitor-driven expansion through terminal differentiation. Analysis by snRNA-seq identified transcript profiles and inferred functions, cell trajectories, signaling interactions, and transcriptional drivers of all but the most highly polyploid cell types of the placenta. These data profile placental development at an unprecedented resolution, provide insights into differentiation and function across time, and provide a resource for future study. |
| Databáze: | OpenAIRE |
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