Volumetric optical mapping in early embryonic hearts using light-sheet microscopy
| Autor: | Shi Gu, Dennis C. Chan, Andrew M. Rollins, Michael W. Jenkins, Pei Ma, Michiko Watanabe |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Heartbeat medicine.diagnostic_test business.industry Cardiac electrophysiology Biology 01 natural sciences Signal Article Atomic and Molecular Physics and Optics law.invention 010309 optics 03 medical and health sciences 030104 developmental biology Optics Optical coherence tomography Confocal microscopy law Light sheet fluorescence microscopy Optical mapping 0103 physical sciences Microscopy medicine business Biotechnology |
| Zdroj: | Biomedical Optics Express. 7:5120 |
| ISSN: | 2156-7085 |
| Popis: | Optical mapping (OM) of electrical activity using voltage-sensitive fluorescent dyes is a powerful tool for the investigation of embryonic cardiac electrophysiology. However, because conventional OM integrates the signal in depth and projects it to a two-dimensional plane, information acquired is incomplete and dependent upon the orientation of the sample. This complicates interpretation of data, especially when comparing one heart to another. To overcome this limitation, we present volumetric OM using light-sheet microscopy, which enables high-speed capture of optically sectioned slices. Voltage-sensitive fluorescence images from multiple planes across entire early embryonic quail hearts were acquired, and complete, orientation-independent, four-dimensional maps of transmembrane potential are demonstrated. Volumetric OM data were collected while using optical pacing to control the heart rate, paving the way for physiological measurements and precise manipulation of the heartbeat in the future. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|