Long noncoding RNA SNHG14 regulates ox-LDL-induced atherosclerosis cell proliferation and apoptosis by targeting miR-186-5p/WIPF2 axis
| Autor: | Cao Zhen, Xiaoming Wang, Dijia Pan, Q Jia, Tao Zhengxian |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Nucleolus Health Toxicology and Mutagenesis Apoptosis Toxicology Flow cytometry 03 medical and health sciences 0302 clinical medicine Western blot medicine Humans Cell Proliferation medicine.diagnostic_test Cell growth Chemistry RNA General Medicine Atherosclerosis Long non-coding RNA Cell biology Lipoproteins LDL MicroRNAs 030104 developmental biology Cell culture 030220 oncology & carcinogenesis RNA Long Noncoding |
| Zdroj: | Humanexperimental toxicology. 40(1) |
| ISSN: | 1477-0903 |
| Popis: | To investigate the role of small nucleolus RNA host gene 14 (SNHG14) in the progression of atherosclerosis (AS), bioinformatics analysis, and other relevant experiments (cell counting kit-8, flow cytometry, quantitative real-time polymerase chain reaction, luciferase reporter, RNA immunoprecipitation, RNA pull-down, and western blot assays) were done. The current study revealed that SNHG14 level was high in the serum of AS patients and oxidized low-density lipoprotein (ox-LDL)-induced AS cell lines. Besides, we found that SNHG14 accelerated cell proliferation while inhibited cell apoptosis in ox-LDL-induced AS cell lines. Next, SNHG14 was confirmed to be a sponge for miR-186-5p in AS cells, and it was validated that SNHG14 regulated AS cell proliferation and apoptosis by sponging miR-186-5p. Moreover, we uncovered that WAS-interacting protein family member 2 (WIPF2) was a downstream target of miR-186-5p in AS cells. Finally, it was demonstrated that miR-186-5p modulated AS cell proliferation and apoptosis via targeting WIPF2. To conclude, our research disclosed that SNHG14 affected ox-LDL-induced AS cell proliferation and apoptosis through miR-186-5p/WIPF2 axis, which may provide a theoretical basis for the treatment and diagnosis of AS. |
| Databáze: | OpenAIRE |
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