Protein Microarray-Guided Development of a Highly Sensitive and Specific Dipstick Assay for Glanders Serodiagnostics
| Autor: | Gabriel E. Wagner, Andreas Berner, Michaela Lipp, Christian Kohler, Karoline Assig, Sabine Lichtenegger, Muhammad Saqib, Elke Müller, Trung T. Trinh, Anne-Marie Gad, Hans Hermann Söffing, Ralf Ehricht, Karine Laroucau, Ivo Steinmetz |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Journal of Clinical Microbiology. 61 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Burkholderia mallei, the causative agent of glanders, is a clonal descendant of Burkholderia pseudomallei, the causative agent of melioidosis, which has lost its environmental reservoir and has a restricted host range. Despite limitations in terms of sensitivity and specificity, complement fixation is still the official diagnostic test for glanders. Therefore, new tools are needed for diagnostics and to study the B. mallei epidemiology. We recently developed a highly sensitive serodiagnostic microarray test for human melioidosis based on the multiplex detection of B. pseudomallei proteins. In this study, we modified our array tests by using anti-horse IgG conjugate and tested sera from B. mallei-infected horses ( |
| Databáze: | OpenAIRE |
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