Differential Gene Expression in High- and Low-Active Inbred Mice
| Autor: | Penny K. Riggs, Tyrone Ceaser, Alicia T. Hamilton, Michelle Dawes, J. Timothy Lightfoot, Trudy Moore-Harrison, Kelli J. Kochan |
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| Rok vydání: | 2014 |
| Předmět: |
Candidate gene
Article Subject lcsh:Medicine Single-nucleotide polymorphism Motor Activity Biology Polymorphism Single Nucleotide General Biochemistry Genetics and Molecular Biology Mice Gene expression Animals Muscle Skeletal Gene Regulation of gene expression Genetics Mice Inbred C3H General Immunology and Microbiology Gene Expression Profiling lcsh:R Haplotype Brain General Medicine Molecular biology Mice Inbred C57BL Gene expression profiling Gene Expression Regulation Haplotypes Regulatory sequence Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2014 (2014) |
| ISSN: | 2314-6141 2314-6133 |
| DOI: | 10.1155/2014/361048 |
| Popis: | Numerous candidate genes have been suggested in the recent literature with proposed roles in regulation of voluntary physical activity, with little evidence of these genes’ functional roles. This study compared the haplotype structure and expression profile in skeletal muscle and brain of inherently high- (C57L/J) and low- (C3H/HeJ) active mice. Expression of nine candidate genes [Actn2,Actn3,Casq1,Drd2,Lepr,Mc4r,Mstn,Papss2, andGlut4(a.k.a.Slc2a4)] was evaluated via RT-qPCR. SNPs were observed in regions ofActn2,Casq1,Drd2,Lepr, andPapss2; however, no SNPs were located in coding sequences or associated with any known regulatory sequences. In mice exposed to a running wheel,Casq1(P=0.0003) andMstn(P=0.002) transcript levels in the soleus were higher in the low-active mice. However, when these genes were evaluated in naïve animals, differential expression was not observed, demonstrating a training effect. Among naïve mice, no genes in either tissue exhibited differential expression between strains. Considering that no obvious SNP mechanisms were determined or differential expression was observed, our results indicate that genomic structural variation or gene expression data alone is not adequate to establish any of these genes’ candidacy or causality in relation to regulation of physical activity. |
| Databáze: | OpenAIRE |
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