Analysis of peptidic epitopes recognized by the three monoclonal antibodies specific for the same region of glycophorin A but showing different properties
| Autor: | Czeslaw Radzikowski, I. Steuden, Danuta Konopińska, Elwira Lisowska, Maria Duk, Kazimiera Waśniowska, Hubert Bartosz-Bechowski, Marcin Czerwinski, Danuta Duś |
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| Rok vydání: | 1992 |
| Předmět: |
Antigenicity
Glycosylation medicine.drug_class Immunology Blotting Western Molecular Sequence Data Dose-Response Relationship Immunologic macromolecular substances Immunodominance Monoclonal antibody Epitope chemistry.chemical_compound Epitopes stomatognathic system Antigen Antibody Specificity medicine Glycophorin Humans Amino Acid Sequence Glycophorins Molecular Biology Peptide sequence Glycoproteins biology Glycopeptides Antibodies Monoclonal Molecular biology Sialic acid Hemagglutinins chemistry biology.protein |
| Zdroj: | Molecular immunology. 29(6) |
| ISSN: | 0161-5890 |
| Popis: | Analysis of epitopes for the three monoclonal antibodies (GPA105, GPA33, OSK4-1) against glycophorin A (GPA) was performed with the use of proteolytic fragments of GPA, the synthetic nonapeptide with the sequence of amino acid residues 35–43 of GPA, and a series of peptides synthesized on plastic pins. The antibodies were specific for a short peptide sequence RAHE (a.a. 39–42 of GPA, MAbs GPA105 and OSK4-1) or RAHEV (a.a. 39–43 of GPA, MAb GPA33). Despite recognizing the same fragment of GPA, the three antibodies showed differences in fine specificity and in response to antigen desialylation. Reactions with single replacement analogs of the RAHEV sequence showed that immunodominant (unreplaceable) residues for the MAbs GPA33 and OSK4-1 were His and Glu, respectively, whereas no such residue was found for the MAb GPA105. Desialylation of the antigen gave strong enhancement of reactivity with the MAb GPA33, moderate —with the MAb GPA105, and weak or no enhancement of reaction with the MAb OSK4-1. The results showed that monoclonal antibodies directed against the same fragment of the polypeptide chain of densely glycosylated antigen may recognize different subsites which are masked at different degree by sialic acid residues. |
| Databáze: | OpenAIRE |
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