A Recombinant Clumping Factor A-Containing Vaccine Induces Functional Antibodies to Staphylococcus aureus That Are Not Observed after Natural Exposure
| Autor: | Kathrin U. Jansen, Srinivas Kodali, Lisa K. McNeil, Ingrid L. Scully, Mininni Terri L, Julio Cesar Hawkins, John H. Vernachio, Elena Severina, Annaliesa S. Anderson, Robert G. K. Donald, Douglas Girgenti, Yury V. Matsuka |
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| Rok vydání: | 2012 |
| Předmět: |
Coagulase
Microbiology (medical) Staphylococcus aureus Clinical Biochemistry Immunology medicine.disease_cause Staphylococcal infections Bacterial Adhesion Virulence factor law.invention Microbiology Mice law medicine Animals Humans Immunology and Allergy Pathogen Mice Inbred BALB C Vaccines biology Fibrinogen Staphylococcal Vaccines Staphylococcal Infections medicine.disease Antibodies Bacterial Virology Clumping factor A biology.protein Recombinant DNA Antibody Protein Binding |
| Zdroj: | Clinical and Vaccine Immunology. 19:1641-1650 |
| ISSN: | 1556-679X 1556-6811 |
| Popis: | Staphylococcus aureus is a Gram-positive pathogen that causes devastating disease and whose pathogenesis is dependent on interactions with host cell factors. Staphylococcal clumping factor A (ClfA) is a highly conserved fibrinogen (Fg)-binding protein and virulence factor that contributes to host tissue adhesion and initiation of infection. ClfA is being investigated as a possible component of a staphylococcal vaccine. We report the development of an Fg-binding assay that is specific for ClfA-mediated binding. Using the assay, we show that despite the presence of anti-ClfA antibodies, human sera from unvaccinated subjects are unable to prevent the binding of S. aureus to an Fg-coated surface. In contrast, antibodies elicited by a recombinant ClfA-containing vaccine were capable of blocking the ClfA-dependent binding of a diverse and clinically relevant collection of staphylococcal strains to Fg. These functional antibodies were also able to displace S. aureus already bound to Fg, suggesting that the ligand-binding activity of ClfA can be effectively neutralized through vaccination. |
| Databáze: | OpenAIRE |
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