Mass spectrometry-based proteomic analysis ofLeishmania donovanisoluble proteins in Indian clinical isolate
| Autor: | Anuradha Dube, Brijesh S. Sisodia, Pragya Misra, Shyam Sundar, Ajit Kumar Shasany, Awanish Kumar |
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| Rok vydání: | 2013 |
| Předmět: |
Proteomics
Microbiology (medical) Drug media_common.quotation_subject Drug Resistance Protozoan Proteins Leishmania donovani India Drug resistance Mass spectrometry Mass Spectrometry Microbiology Pathogenesis parasitic diseases medicine Humans Immunology and Allergy Leishmaniasis Vaccines media_common Gel electrophoresis General Immunology and Microbiology biology General Medicine biology.organism_classification Leishmania medicine.disease Molecular biology Infectious Diseases Visceral leishmaniasis Leishmaniasis Visceral |
| Zdroj: | Pathogens and Disease. 70:84-87 |
| ISSN: | 2049-632X |
| Popis: | Leishmania donovani, a causative organism of visceral leishmaniasis (VL), is responsible for high mortality throughout the world. Due to the unsatisfactory treatment measures and increasing drug resistance, there has been an urgent need to develop novel drug/vaccine targets against VL. The aim of this study was to identify novel targets in soluble L. donovani (SLD) protein. SLD protein was isolated and resolved by two-dimensional gel electrophoresis and analyzed through MALDI-TOF/TOF-based mass spectrometry. Proteomic results identified several proteins as drug targets, Th1 stimulatory, novel, and hypothetical proteins which could have crucial biological functions in Leishmania pathogenesis. |
| Databáze: | OpenAIRE |
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