Comprehensive Proteomic Profiling of Pressure Ulcers in Patients with Spinal Cord Injury Identifies a Specific Protein Pattern of Pathology
| Autor: | Montserrat Baldan-Martin, Maria G. Barderas, Nerea Corbacho-Alonso, Juan Antonio López, Laura Mourino-Alvarez, Tamara Sastre-Oliva, Felix Gil-Dones, Tatiana Martin-Rojas, Jesús Vázquez, Jose Manuel Arevalo |
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| Přispěvatelé: | Instituto de Salud Carlos III, European Regional Development Fund, Sociedad Española de Cardiología |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Male Proteomics Pathology medicine.medical_specialty Apolipoprotein B Complement factor I Spinal cord injury Critical Care and Intensive Care Medicine Tandem mass tag Pressure ulcer Technology Advances Pathogenesis 030207 dermatology & venereal diseases 03 medical and health sciences Young Adult 0302 clinical medicine Risk Factors medicine Tandem mass tags Humans Spinal Cord Injuries Aged Pressure Ulcer Wound Healing biology business.industry Proteomic Profiling Membrane Proteins Middle Aged medicine.disease spinal cord injury Fibronectin tandem mass tags 030104 developmental biology Emergency Medicine biology.protein Intercellular Signaling Peptides and Proteins Female business Chromatography Liquid |
| Zdroj: | Advances in Wound Care Repisalud Instituto de Salud Carlos III (ISCIII) |
| ISSN: | 2162-1934 2162-1918 |
| Popis: | Objective: Severe pressure ulcers (PUs) do not respond to conservative wound therapy and need surgical repair. To better understand the pathogenesis and to advance on new therapeutic options, we focused on the proteomic analysis of PU, which offers substantial opportunities to identify significant changes in protein abundance during the course of PU formation in an unbiased manner. Approach: To better define the protein pattern of this pathology, we performed a proteomic approach in which we compare severe PU tissue from spinal cord injury (SCI) patients with control tissue from the same patients. Results: We found 76 proteins with difference in abundance. Of these, 10 proteins were verified as proteins that define the pathology: antithrombin-III, alpha-1-antitrypsin, kininogen-1, alpha-2-macroglobulin, fibronectin, apolipoprotein A-I, collagen alpha-1 (XII) chain, haptoglobin, apolipoprotein B-100, and complement factor B. Innovation: This is the first study to analyze differential abundance protein of PU tissue from SCI patients using high-throughput protein identification and quantification by tandem mass tags followed by liquid chromatography tandem mass spectrometry. Conclusion: Differential abundance proteins are mainly involved in tissue regeneration. These proteins might be considered as future therapeutic options to enhance the physiological response and permit cellular repair of damaged tissue. This work was supported by grants from the Instituto de Salud Carlos III (PI14/01917, PI18/00995, PT13/0001/0013) for Funding: ISCIII (‘‘PI14/01917, PI18/00995, PT13/0001/0013)’’, co-funded by ERDF/ESF, ‘‘Investing in your future’’. Redes Tema ticas de Investigacion Cooperativa (FONDOS FEDER, RD12/0042/0071). Sociedad Espanola de Cardiologıa para la Investigacion Basica 2017. Grant PRB3 (IPT17/0019—ISCIII-SGEFI/ERDF). These results are aligned with the Spanish initiative on the Human Proteome Project (SpHPP). Sí |
| Databáze: | OpenAIRE |
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