Prediction of Spontaneous Regression of Cervical Intraepithelial Neoplasia Lesions Grades 2 and 3 by Proteomic Analysis
| Autor: | Emiel A. M. Janssen, Bianca van Diermen, Kai-Erik Uleberg, Jan P. A. Baak, Cato Brede, Irene T Øvestad, Einar Gudlaugsson, Anne Hjelle, Ane Cecilie Munk |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Zinc finger
Pathology medicine.medical_specialty medicine.diagnostic_test Article Subject business.industry Cervical intraepithelial neoplasia medicine.disease Biochemistry Regression female genital diseases and pregnancy complications Persistence (computer science) Text mining Biopsy Medicine Immunohistochemistry business Molecular Biology Identification criteria Research Article |
| Zdroj: | International Journal of Proteomics |
| ISSN: | 2090-2166 |
| DOI: | 10.1155/2014/129064 |
| Popis: | Regression of cervical intraepithelial neoplasia (CIN) 2-3 to CIN 1 or less is associated with immune response as demonstrated by immunohistochemistry in formaldehyde-fixed paraffin-embedded (FFPE) biopsies. Proteomic analysis of water-soluble proteins in supernatants of biopsy samples with LC-MS (LTQ-Orbitrap) was used to identify proteins predictive of CIN2-3 lesions regression. CIN2-3 in the biopsies and persistence (CIN2-3) or regression (≤CIN1) in follow-up cone biopsies was validated histologically by two experienced pathologists. In a learning set of 20 CIN2-3 (10 regressions and 10 persistence cases), supernatants were depleted of seven high abundance proteins prior to unidimensional LC-MS/MS protein analysis. Mean protein concentration was 0.81 mg/mL (range: 0.55–1.14). Multivariate statistical methods were used to identify proteins that were able to discriminate between regressive and persistent CIN2-3. The findings were validated in an independent test set of 20 CIN2-3 (10 regressions and 10 persistence cases). Multistep identification criteria identified 165 proteins. In the learning set, zinc finger protein 441 and phospholipase D6 independently discriminated between regressive and persistent CIN2-3 lesions and correctly classified all 20 patients. Nine regression and all persistence cases were correctly classified in the validation set. Zinc finger protein 441 and phospholipase D6 in supernatant samples detected by LTQ-Orbitrap can predict regression of CIN2-3. |
| Databáze: | OpenAIRE |
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