Universal microfluidic platform for bioassays in anchored droplets
| Autor: | Gabriel Amselem, Charles N. Baroud, Sébastien Michelin, Cyprien Guermonprez, Benoît Drogue |
|---|---|
| Přispěvatelé: | Laboratoire d'hydrodynamique (LadHyX), École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
education.field_of_study
Materials science medicine.diagnostic_test [PHYS.MECA.MEFL]Physics [physics]/Mechanics [physics]/Mechanics of the fluids [physics.class-ph] 010401 analytical chemistry Population Microfluidics Biomedical Engineering Bioengineering Nanotechnology 02 engineering and technology General Chemistry 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry 6. Clean water 0104 chemical sciences Antibiogram Lab-On-A-Chip Devices Self-healing hydrogels medicine Biological Assay 0210 nano-technology education ComputingMilieux_MISCELLANEOUS |
| Zdroj: | Lab on a Chip Lab on a Chip, Royal Society of Chemistry, 2016, 16 (21), pp.4200-4211. ⟨10.1039/C6LC00968A⟩ |
| ISSN: | 1473-0197 1473-0189 |
| Popis: | In spite of the large number of droplet-based microfluidic tools that have appeared in recent years, their penetration into non-specialist labs remains limited to a small number of applications. This is partly due to the lack of a generic platform that integrates all of the necessary operations for end-users, and partly to the increasing complexity that emerges as several operations are combined together. Here we report the development of a platform that provides the capabilities of multiwell plates in a two-dimensional array of nanoliter droplets: encapsulation, time-resolved monitoring and variation of well contents, as well as the ability to selectively extract the contents of any of the wells. We demonstrate these capabilities by encapsulating thousands of individual bacterial cells in droplets that are stored on a two-dimensional array of surface-energy anchors. Bacterial culture can be performed either in liquid or hydrogel droplets, both of which allow precise quantification using either standard measurements or digital enumeration. Using hydrogels allows the removal of the external oil that surrounds the aqueous drops, for instance in order to apply a gradient of antibiotics across the droplet population. This defines a protocol to obtain an antibiogram in a single experiment. Finally, the liquid to gel transition provides a robust way to selectively extract any droplet from the array, by melting it with a focused laser. When combined with further off-chip culture or genotyping, this platform provides a unique culturing environment to relate phenotype and genotype measurements on monoclonal colonies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|