An aqueous pomegranate peel extract (Punica granatum) protect against Elastase-induced pulmonary emphysema in Sprague Dawley rats model
| Autor: | Herráez Thomas Pherraez, Martín-Barrasa Jose Luis, Espinosa De Los Monteros Y Zaya Antonio, Bachoual Rafik, Ramos-Nuez Ángela, Zioud Fatma, González Martín Jesús María, Santana Reyes Laura |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Punica granatum
Lung Lung inflammation medicine.diagnostic_test Chemistry medicine.medical_treatment Elastase Intraperitoneal injection Inflammation Pharmacology CCL2 respiratory system RS1-441 Bronchoalveolar lavage medicine.anatomical_structure emphysema Pharmacy and materia medica medicine Rat Platelet Tumor necrosis factor alpha medicine.symptom Lung oedema |
| Zdroj: | Brazilian Journal of Pharmaceutical Sciences, Vol 57 (2021) Brazilian Journal of Pharmaceutical Sciences, Volume: 57, Article number: e18972, Published: 26 NOV 2021 |
| ISSN: | 2175-9790 |
| Popis: | We investigated the effect of Punica granatum peel aqueous extract (PGE), on pulmonary inflammation and alveolar degradation induced by intratracheal administration of Elastase in Sprague Dawley rats. Lung inflammation was induced in rats by intratracheal instillation of Elastase. On day 1 and 2, animals received an intraperitoneal injection of PGE (200 mg/mL), three hours later, they were intratracheally instilled with 25U/kg pancreatic porcine Elastase. Animals were sacrificed 7 days later. Bronchoalveolar lavage (BAL) were collected and cellularity, histology and mRNA expression of Monocyte chemotactic protein 1(MCP-1), Tumor Necrosis Factor-Alpha (TNF-α), Interleukin 6 (IL-6), and Matrix Metalloproteinase-2 (MMP-2) were studied. In addition, activity of TNF- α, IL-6 and MCP-1 on BAL were also analyzed by ELISA Kit. Elastase administration increased: BAL cellularity, neutrophils recruitment and BAL MCP1, IL-6 expressions. It also increased lung TNF-α, MCP-1, MMP-2 expressions, platelets recruitment, histological parameters at 7th day of elastase treatment. Intraperitoneal injection of 200 mg/kg of PGE reduced, significantly, BAL cellularity, and neutrophils recruitment. However, in animal treated with PGE, MCP-1, MMP-2 and IL-6 on day 7, were similar to the Sham group. Treatment with PGE (200 mg/ kg) also significantly reduced lung TNF-α, and MCP-1 expression. This study reveals that PGE Punica granatum protects against elastase lung inflammation and alveolar degradation induced in rats. |
| Databáze: | OpenAIRE |
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