Regulation of polymerase II transcription by 7SK snRNA: two distinct RNA elements direct P-TEFb and HEXIM1 binding
| Autor: | Sylvain Egloff, Elodie Van Herreweghe, Tamás Kiss |
|---|---|
| Přispěvatelé: | Laboratoire de biologie moléculaire eucaryote (LBME), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre de Biologie Intégrative (CBI), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
5' Flanking Region
RNA polymerase II MESH: Base Sequence MESH: RNA Small Nuclear 0302 clinical medicine RNA Small Nuclear Positive Transcriptional Elongation Factor B 3' Flanking Region Phosphorylation RNA polymerase II holoenzyme Conserved Sequence 0303 health sciences MESH: Conserved Sequence biology General transcription factor RNA-Binding Proteins Articles MESH: Positive Transcriptional Elongation Factor B 3. Good health MESH: Nucleic Acid Conformation 030220 oncology & carcinogenesis Transcription factor II F Transcription factor II E RNA Polymerase II Transcription factor II D Transcription factor II B Protein Binding Transcriptional Activation Molecular Sequence Data MESH: 3' Flanking Region Cell Line 03 medical and health sciences Humans MESH: Protein Binding [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Molecular Biology 030304 developmental biology MESH: 5' Flanking Region MESH: Humans MESH: Molecular Sequence Data Base Sequence MESH: Phosphorylation Cell Biology Molecular biology MESH: RNA Polymerase II MESH: Cell Line MESH: RNA-Binding Proteins MESH: Trans-Activation (Genetics) biology.protein Nucleic Acid Conformation Small nuclear RNA Transcription Factors |
| Zdroj: | Molecular and Cellular Biology Molecular and Cellular Biology, American Society for Microbiology, 2006, 26, pp.630-642 Molecular and Cellular Biology, American Society for Microbiology, 2006, 26 (2), pp.630-42. ⟨10.1128/MCB.26.2.630-642.2006⟩ |
| ISSN: | 0270-7306 1098-5549 |
| DOI: | 10.1128/MCB.26.2.630-642.2006⟩ |
| Popis: | The positive transcription elongation factor b (P-TEFb), a complex of Cdk9 and cyclin T1/T2, stimulates transcription by phosphorylating RNA polymerase II. The 7SK small nuclear RNA, in cooperation with HEXIM1 protein, functions as a general polymerase II transcription regulator by sequestering P-TEFb into a large kinase-inactive 7SK/HEXIM1/P-TEFb complex. Here, determination and characterization of the functionally essential elements of human 7SK snRNA directing HEXIM1 and P-TEFb binding led to a new model for the assembly of the 7SK/HEXIM1/P-TEFb regulatory complex. We demonstrate that two structurally and functionally distinct protein binding elements located in the 5'- and 3'-terminal hairpins of 7SK support the in vivo recruitment of HEXIM1 and P-TEFb. Consistently, a minimal regulatory RNA composed of the 5' and 3' hairpins of 7SK can modulate polymerase II transcription in HeLa cells. HEXIM1 binds independently and specifically to the G24-C48/G60-C87 distal segment of the 5' hairpin of 7SK. Binding of HEXIM1 is a prerequisite for association of P-TEFb with the G302-C324 apical region of the 3' hairpin of 7SK that is highly reminiscent of the human immunodeficiency virus transactivation-responsive RNA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|