Effect of activin-A on progesterone synthesis in human luteal cells
| Autor: | Alessandro Caruso, Antonio Lanzone, Nicoletta Di Simone, Felice Petraglia, E. Ronsisvalle, Salvatore Mancuso |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Adult
medicine.medical_specialty Cell type endocrine system Cell ACTIVIN-A. luteal phase infertility Luteal phase Biology Chorionic Gonadotropin Activin corpus luteum luteal cells progesterone Basal (phylogenetics) Luteal Cells Corpus Luteum Internal medicine medicine Humans Inhibins reproductive and urinary physiology Cells Cultured Progesterone urogenital system Obstetrics and Gynecology Middle Aged Activins medicine.anatomical_structure Endocrinology ACTIVIN-A Settore MED/40 - GINECOLOGIA E OSTETRICIA Reproductive Medicine Cell culture Collagenase Female Corpus luteum hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0015-0282 |
| Popis: | Objective To examine the effect of activin-A on basal and hCG-stimulated P production by human luteal cells. Design Mixed luteal cell cultures and distinct cultures of two luteal cell types: small and large luteal cells from early and midluteal phase. Setting Corpora lutea (CL) were obtained from the Obstetrics and Gynecology Department of the Catholic University, Rome, Italy. Patients Fifteen nonpregnant women between 30 and 45 years of age underwent surgery for nonendocrine gynecological diseases. Interventions Corpora lutea were obtained at the time of hysterectomy. The luteal cells were dispersed in Ham's F-12 medium containing collagenase at 37°C in shaking water bath for 2 hours, filtered, centrifuged, and resuspended in fresh medium. Main Outcome Measures The cells diluted to a final concentration of 60,000 to 100,000 cells/mL of medium. After 24 hours, the cells attached to the wells and were incubated with or without hCG and/or activin-A at different concentrations. Results Activin-A starting from 25 μ g/L significantly decreased basal and hCG (250 mIU/mL [conversion to SI unit, 1.00])-induced P production by mixed luteal cells. The small luteal cells responded to hCG (250 mIU/mL), and the treatment with activin-A (from 25 to 100 μ g/L) reduced their basal and hCG-induced P production. Activin-A addition did not change the amount of P release by large luteal cells at any concentration. Conclusions These results imply that activin-A plays a role in the local regulation of human CL. |
| Databáze: | OpenAIRE |
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