BCG infection in mice is promoted by naïve mesenchymal stromal cells (MSC) and suppressed by poly(A:U)-conditioned MSC
| Autor: | Andrey G. Cherednichenko, Yakov Sh. Schwartz, Sergey N. Belogorodtsev, Sergey Pustylnikov, Pavel N. Filimonov, Vladimir Krasnov |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Male Pathology medicine.medical_specialty Immunology Spleen Mesenchymal Stem Cell Transplantation Microbiology Regenerative medicine Immunophenotyping Cell therapy 03 medical and health sciences 0302 clinical medicine medicine Animals Tuberculosis Mycobacterium bovis Mice Inbred BALB C biology Mesenchymal stem cell Mesenchymal Stem Cells medicine.disease biology.organism_classification Bacterial Load Transplantation 030104 developmental biology Infectious Diseases medicine.anatomical_structure Granuloma Culture Media Conditioned Host-Pathogen Interactions Cytokines Inflammation Mediators 030215 immunology Polyribonucleotides |
| Zdroj: | Tuberculosis (Edinburgh, Scotland). 101 |
| ISSN: | 1873-281X |
| Popis: | Mesenchymal stromal cells (MSC) transplantation is an actively studied therapeutic approach used in regenerative medicine and in the field of control of immunoinflammatory response. Conditioning of MSC in culture can form their predominantly pro- or anti-inflammatory phenotypes. We demonstrated that poly(A:U)-conditioning of bone marrow-derived mouse MSC induced predominantly pro-inflammatory phenotype. The effects of administration of naive MSC (nMSC) or conditioned MSC (cMSC) on the course of mycobacterial infection were studied. BALB/c mice infected i.p. with 5 × 106 M. bovis BCG were successively injected i.v. with 0.75 × 106 of nMSC or cMSC in 11 and 12.5 weeks after infection and sacrificed at the week 14. Histological and bacteriological examination of BCG-infected animals revealed low bacterial loads in liver, lungs and spleen; the bacterial load in spleen was higher than in other organs. Treatment with nMSC induced 3-fold increase of the number of bacteria in spleen granulomas, while cMSC decreased significantly the number of bacteria in BCG-positive granulomas. Analysis of preparations of organ homogenates by luminescent microscopy, MGIT cultures and CFU count on Lowenstein-Jensen medium revealed that nMSC promoted mycobacterial growth whereas cMSC suppressed mycobacterial growth significantly. We concluded that MSC therapy can be effective in mycobacterial infection, but only in a case of appropriate conditioning of the cells. |
| Databáze: | OpenAIRE |
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