Adipose-derived stem cells combined with inorganic bovine bone in calvarial bone healing in rats with type 2 diabetes
| Autor: | Jianhui Zhao, Mingqun Qin, Long Li, Naibin Liu, Chao Xie, Lihua Liang, Dehua Li, Yingliang Song, Dao-Cai Sun, Tiejun Jiao, Yan Liu, Guike Zou |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Pathology Bone Regeneration Time Factors medicine.medical_treatment Population Cell Culture Techniques Adipose tissue Bone healing Bone grafting Diet High-Fat Streptozocin Diabetes Mellitus Experimental Rats Sprague-Dawley Random Allocation Bone Density Osteogenesis medicine Animals education Bone regeneration Cell Proliferation education.field_of_study Tissue Scaffolds business.industry Stem Cells Skull Membranes Artificial X-Ray Microtomography Streptozotocin Surgery Rats Treatment Outcome Adipose Tissue Diabetes Mellitus Type 2 Bone Substitutes Microscopy Electron Scanning Periodontics Cattle Implant Stem cell Bone Diseases business medicine.drug |
| Zdroj: | Journal of periodontology. 85(4) |
| ISSN: | 1943-3670 |
| Popis: | Clinical studies have revealed that patients with type 2 diabetes mellitus (DM) have higher implant and bone grafting failure rates than the general population, likely owing to inferior bone healing. The authors sought to investigate whether adipose-derived stem cells (ASCs) combined with inorganic bovine bone improves bone repair in calvarial vertical critical-sized defects (CSDs) in rats with type 2 DM.Bovine bone alone or seeded with 3 × 10(5), 3 × 10(6), or 3 × 10(7) ASCs/graft was randomly transplanted into calvarial CSDs in rats with DM induced by a high-fat diet with low-dose streptozotocin. Specimens were assayed using microcomputed tomography and histomorphometry at 4 and 8 weeks postimplantation.The histologic results showed an increase in new bone formation in the experimental groups compared with the control group. Both bone volume/total volume and trabecular thickness of newly formed bone within CSDs were the highest, and trabecular spacing was the lowest, in the 3 × 10(6) group at 8 weeks for the most favorable outcome. The results showed that the amount of new bone was greatest in the 3 × 10(6) group by 8 weeks.ASCs enhanced vertical bone regeneration in calvarial defects in rats with type 2 DM, when used in association with bovine bone scaffolds. The findings suggest that a combination of ASCs and bovine bone scaffolds could improve bone quantity in vertical bone defects. |
| Databáze: | OpenAIRE |
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