Analysis of STAT1 expression and biological activity reveals interferon-tau-dependent STAT1-regulated SOCS genes in the bovine endometrium
| Autor: | Gareth D. Healey, Caroline Eozenou, A. Vitorino Carvalho, Olivier Sandra, Pierrette Reinaud, Patrick Lonergan, Nathalie Rodde, Niamh Forde, M Leveugle, C Giraud Delville, Geneviève Jolivet, Laurence Gall, Christophe Richard, Iain Martin Sheldon, Martine Chebrout, A Lesage Padilla |
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| Přispěvatelé: | Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Swansea University, School of Agriculture and Food Science, University College Dublin [Dublin] (UCD), Centre National de Ressources Génomiques Végétales (CNRGV), Institut National de la Recherche Agronomique (INRA), Unité Mathématique Informatique et Génome (MIG), European Network of Excellence ‘Embryo Implantation Control’ (EMBIC, LSHN-CT-2004–512040, ANR-08-GENM-037, AIP Bioressources CHIPSTRAFFIC, Science Foundation Ireland and the Higher Education Authority Irish Centre for High-End Computing (ICHEC) and the UK Biotechnology and Biological Sciences Research Council (BBSRC, BB/I017240/1), ANR-08-GENM-0037,MEETAC,Valorisation des données transcriptomiques bovines à l'interface mère-embryon pour une compréhension intégrée de l'implantation(2008), European Project: 28817,EMBIC, École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA) |
| Rok vydání: | 2014 |
| Předmět: |
0301 basic medicine
Time Factors Suppressor of Cytokine Signaling Proteins Pregnancy Proteins Endometrium Endocrinology Pregnancy Gene expression implantation STAT1 SOCS3 Phosphorylation Promoter Regions Genetic [SDV.BDD]Life Sciences [q-bio]/Development Biology Cells Cultured Insemination Artificial transcription factor Regulation of gene expression Cell biology STAT1 Transcription Factor medicine.anatomical_structure Interferon Type I Female Signal Transduction Biotechnology medicine.medical_specialty Primary Cell Culture Active Transport Cell Nucleus Estrous Cycle Gestational Age chromatin immunoprecipitation Biology 03 medical and health sciences Internal medicine Genetics medicine Animals Embryo Implantation Molecular Biology Binding Sites uterus Suppressor of cytokine signaling 1 [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology Interferon tau 030104 developmental biology IRF1 Gene Expression Regulation Reproductive Medicine cattle biology.protein Animal Science and Zoology Developmental Biology |
| Zdroj: | Reproduction, Fertility and Development Reproduction, Fertility and Development, CSIRO Publishing, 2016, ⟨10.1071/RD14034⟩ Europe PubMed Central |
| ISSN: | 1031-3613 |
| DOI: | 10.1071/RD14034⟩ |
| Popis: | A. Vitorino Carvalho is a PhD recipient from the French Ministry for Teaching and Research – Doctoral School ED419 Université Paris Sud. Remerciements au personnel des unités expérimentales de l'INRA (Avord, France, Saint-Genès-Champanelle, France; Nouzilly, France) pour la gestion des animaux et le recueil de tissu; International audience; Signal transducer and activator of transcription (STAT) proteins are critical for the regulation of numerous biological processes. In cattle, microarray analyses identified STAT1 as a differentially expressed gene in the endometrium during the peri-implantation period. To gain new insights about STAT1 during the oestrous cycle and early pregnancy, we investigated STAT1 transcript and protein expression, as well as its biological activity in bovine tissue and cells of endometrial origin. Pregnancy increased STAT1 expression on Day 16, and protein and phosphorylation levels on Day 20. In cyclic and pregnant females, STAT1 was located in endometrial cells but not in the luminal epithelium at Day 20 of pregnancy. The expression of STAT1 during the oestrous cycle was not affected by progesterone supplementation. In vivo and in vitro, interferon-tau (IFNT) stimulated STAT1 mRNA expression, protein tyrosine phosphorylation and nuclear translocation. Using chromatin immunoprecipitation in IFNT-stimulated endometrial cells, we demonstrated an increase of STAT1 binding on interferon regulatory factor 1 (IRF1), cytokine-inducible SH2-containing protein (CISH), suppressor of cytokine signaling 1 and 3 (SOCS1, SOCS3) gene promoters consistent with the induction of their transcripts. Our data provide novel molecular insights into the biological functions of STAT1 in the various cells composing the endometrium during maternal pregnancy recognition and implantation. |
| Databáze: | OpenAIRE |
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