Transcription factor FvTCP9 promotes strawberry fruit ripening by regulating the biosynthesis of abscisic acid and anthocyanins
| Autor: | Ying-Qiang Wen, Yi-Jie Gou, Jie Liu, Wei Wei, Dong Zhu, Jia-Yue Feng, Yin-Ge Xie, Pin-Pin Bi, Yang-Yang Ma, Yang Hu |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Plant Science 01 natural sciences Fragaria Anthocyanins 03 medical and health sciences chemistry.chemical_compound Woodland Strawberry Gene Expression Regulation Plant Genetics Gene silencing Gene Transcription factor Abscisic acid Plant Proteins biology fungi food and beverages Ripening biology.organism_classification Horticulture 030104 developmental biology chemistry Anthocyanin Fruit 010606 plant biology & botany Abscisic Acid Transcription Factors |
| Zdroj: | Plant physiology and biochemistry : PPB. 146 |
| ISSN: | 1873-2690 |
| Popis: | The plant-specific transcription factor TEOSINTE BRANCHED 1, CYCLOIDEA, and PROLIFERATING4 CELL FACTORS (TCP) plays a crucial role in plant growth and development. However, there have been no studies reporting on the function of strawberry TCP in regulating fruit development. In this study, FvTCP9, a woodland strawberry (Fragaria vesca) TCP gene, was isolated to explore its function in fruit ripening. The transcript accumulation levels of FvTCP9 were high in fruits, specifically in red fruits compared with other tissues or organs. Transient expression of the FvTCP9 gene in cultivated strawberry fruits revealed that over-expression of FvTCP9 promoted fruit ripening. Meanwhile, silencing FvTCP9, using tobacco rattle virus-induced gene silencing (VIGS), inhibited fruit ripening. The changes in ripening-related physiological conditions in transient fruits, such as the accumulation of anthocyanins and abscisic acid (ABA), and fruit firmness confirmed above results. Results suggested that FvTCP9 was involved in the biosynthesis of ABA and anthocyanins to regulate fruit ripening. Transcription analysis showed that the expression levels of ABA signaling-related genes (FaNCED1, FaPYR1, FaSnRK2, and FaABI5) were affected by FvTCP9. A yeast two-hybrid assay revealed that FvTCP9 interacted physically with FaMYC1 to modulate the biosynthesis process of anthocyanins. Taken together, this study demonstrated that FvTCP9 promoted fruit ripening by regulating the biosynthesis of ABA and anthocyanins. |
| Databáze: | OpenAIRE |
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