Presentation of protective antigen to the mouse immune system: immune sequelae

Autor: Allan Bennett, R. J. Beedham, S. D. Perkins, Julie Miller, E. D. Williamson, Les Baillie
Rok vydání: 1999
Předmět:
Zdroj: Journal of Applied Microbiology. 87:315-317
ISSN: 1365-2672
1364-5072
DOI: 10.1046/j.1365-2672.1999.00901.x
Popis: Protective antigen (PA), the major protective component of the existing vaccine, is a potent immunogen. Protective antigen in alhydrogel induced a high serum IgG titre (> log10 4) in both the C57B16 and Balb/c mouse and the predominant subclass of antibody induced was IgG1, indicating that the response to PA was predominantly Th2 directed. When plasmid DNA encoding PA was used to immunize the Balb/c mouse, a low serum IgG titre was detected (≤log10 1), which was slightly increased by boosting with plasmid DNA. However, when mice immunized with plasmid DNA were later boosted with rPA, a significant and rapid increase in titre (up to threefold) was observed. Priming mice with PA-encoding plasmid DNA may be a mechanism of enhancing and accelerating the immune response to PA.
Databáze: OpenAIRE