p53-induced microRNA-1246 inhibits the cell growth of human hepatocellular carcinoma cells by targeting NFIB
| Autor: | Liye Cao, Ai-Min Zhang, Yong Li, Quan Zhang, Xiao-Shi Jin, Shujie Cheng |
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| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
Carcinoma Hepatocellular Cell Apoptosis Biology medicine.disease_cause Cell Line Tumor microRNA medicine Humans RNA Small Interfering Cell Proliferation Oncogene Cell growth Liver Neoplasms General Medicine Hep G2 Cells Cell cycle digestive system diseases Gene Expression Regulation Neoplastic MicroRNAs NFI Transcription Factors medicine.anatomical_structure Cell Transformation Neoplastic Oncology NFIB Cancer research RNA Interference Tumor Suppressor Protein p53 Carcinogenesis A431 cells |
| Zdroj: | Oncology reports. 33(3) |
| ISSN: | 1791-2431 |
| Popis: | In recent years, miR-1246 has been identified as a transcriptional target of p53 in Down syndrome and may provide a new p53-miR-1246-DYRK1A-NFAT pathway in cancer. The present study aimed to explore the role of miR-1246 in the tumorigenesis of human hepatocellular carcinoma (HCC). We found that wild-type p53 regulated the expression of miR-1246 in HCC cell lines, and alteration of miR-1246 modulated cell proliferation, colony formation ability and apoptosis. The nuclear factor I/B (NFIB), an oncogene, was identified as a direct target gene of miR-1246 using a fluorescent reporter assay. Overexpression of NFIB abolished the regulation of cell apoptosis caused by miR-1246 in HepG2 cells. This finding suggests that miR-1246 is regulated by p53 and suppresses the growth of human HCC by targeting NFIB. Here, we propose a new p53-miR-1246-NFIB pathway in HCC. |
| Databáze: | OpenAIRE |
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