Imaging flow cytometry assays for quantifying pigment grade titanium dioxide particle internalization and interactions with immune cells in whole blood
Autor: | Jonathan J. Powell, Nuno Faria, Rachel E. Hewitt, Laetitia C. Pele, Bradley Vis |
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Přispěvatelé: | Hewitt, Rachel [0000-0002-2367-1822], Powell, Jonathan [0000-0003-2738-1715], Apollo - University of Cambridge Repository |
Rok vydání: | 2017 |
Předmět: |
lymphocytes
0301 basic medicine membrane adherence Histology media_common.quotation_subject 02 engineering and technology Peripheral blood mononuclear cell Pathology and Forensic Medicine Flow cytometry 03 medical and health sciences chemistry.chemical_compound Immune system neutrophils In vivo medicine Humans Particle Size Coloring Agents Internalization Cells Cultured Whole blood media_common Titanium medicine.diagnostic_test Chemistry titanium dioxide particles whole blood Original Articles Cell Biology imaging flow cytometry Flow Cytometry 021001 nanoscience & nanotechnology internalization 030104 developmental biology Titanium dioxide Immunology darkfield Leukocytes Mononuclear Biophysics Original Article nanoparticles monocytes 0210 nano-technology Cytometry |
Zdroj: | Cytometry |
ISSN: | 1552-4922 |
Popis: | Pigment grade titanium dioxide is composed of sub‐micron sized particles, including a nanofraction, and is widely utilized in food, cosmetic, pharmaceutical, and biomedical industries. Oral exposure to pigment grade titanium dioxide results in at least some material entering the circulation in humans, although subsequent interactions with blood immune cells are unknown. Pigment grade titanium dioxide is employed for its strong light scattering properties, and this work exploited that attribute to determine whether single cell–particle associations could be determined in immune cells of human whole blood at “real life” concentrations. In vitro assays, initially using isolated peripheral blood mononuclear cells, identified titanium dioxide associated with the surface of, and within, immune cells by darkfield reflectance in imaging flow cytometry. This was confirmed at the population level by side scatter measurements using conventional flow cytometry. Next, it was demonstrated that imaging flow cytometry could quantify titanium dioxide particle‐bearing cells, within the immune cell populations of fresh whole blood, down to titanium dioxide levels of 10 parts per billion, which is in the range anticipated for human blood following titanium dioxide ingestion. Moreover, surface association and internal localization of titanium dioxide particles could be discriminated in the assays. Overall, results showed that in addition to the anticipated activity of blood monocytes internalizing titanium dioxide particles, neutrophil internalization and cell membrane adhesion also occurred, the latter for both phagocytic and nonphagocytic cell types. What happens in vivo and whether this contributes to activation of one or more of these different cells types in blood merits further attention. © 2017 The Authors. Cytometry Part A Published by Wiley Periodicals, Inc. on behalf of ISAC. |
Databáze: | OpenAIRE |
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