Phenotypic and molecular characterisations of carbapenem-resistant Acinetobacter baumannii strains isolated in Madagascar

Autor: Jean-Marc Collard, Carole Kowalewicz, Frédérique Randrianirina, Andriniaina Rakotondrasoa, Elisoa Hariniaina Ratsima, Pierrette Landrie Simo Tchuinte, Mamitiana Alain Noah Rabenandrasana, Zafitsara Zo Andrianirina, Vincent Enouf, Volasoa Herilalaina Andrianoelina
Přispěvatelé: Institut Pasteur de Madagascar, Réseau International des Instituts Pasteur (RIIP), Centre Hospitalier de Soavinandriana (CENHOSOA), Pasteur International Bioresources network (PIBNet), Institut Pasteur [Paris], This study is based upon a postdoctoral internship supported by Postdoctoral Grant CALMETTE and YERSIN from Institut Pasteur Department of International Affairs., Institut Pasteur [Paris] (IP)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Acinetobacter baumannii
Drug resistance
Antimicrobial resistance
MESH: Carbapenems
MESH: Madagascar
Plasmid
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Genotype
polycyclic compounds
Medicine
MESH: Genomic Islands
Pharmacology (medical)
Insertion sequence
MESH: Bacterial Proteins
0303 health sciences
MESH: Microbial Sensitivity Tests
biology
3. Good health
MESH: Integrons
Infectious Diseases
MESH: DNA Transposable Elements
MESH: Acinetobacter Infections
Microbiology (medical)
Virulence
MESH: Phenotype
lcsh:Infectious and parasitic diseases
Microbiology
03 medical and health sciences
Antibiotic resistance
MESH: Plasmids
Intensive care
MESH: Anti-Bacterial Agents
lcsh:RC109-216
030304 developmental biology
MESH: Virulence Factors
MESH: Humans
030306 microbiology
business.industry
MESH: Acinetobacter baumannii
Public Health
Environmental and Occupational Health
MESH: Drug Resistance
Multiple
Bacterial
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
biology.organism_classification
Carbapenem-resistance
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
bacteria
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
business
Zdroj: Antimicrobial Resistance and Infection Control
Antimicrobial Resistance and Infection Control, BioMed Central, 2019, 8 (1), pp.31. ⟨10.1186/s13756-019-0491-9⟩
Antimicrobial Resistance and Infection Control, 2019, 8 (1), pp.31. ⟨10.1186/s13756-019-0491-9⟩
Antimicrobial Resistance and Infection Control, Vol 8, Iss 1, Pp 1-9 (2019)
ISSN: 2047-2994
DOI: 10.1186/s13756-019-0491-9⟩
Popis: Background The present study aimed to perform a deep phenotypic and genotypic analysis of 15 clinical carbapenem-resistant Acinetobacter baumannii (CRAb) strains isolated in Madagascar between 2008 and 2016 from diverse sources. Methods CRAb isolates collected from the Clinical Biology Centre of the Institut Pasteur of Madagascar, from the neonatal unit of Antananarivo military hospital, and from intensive care units of Mahajanga Androva and Antananarivo Joseph Ravoahangy Andrianavalona (HJRA) hospitals were subjected to susceptibility testing. Whole-genome sequencing allowed us to assess the presence of antibiotic-resistance determinants, insertion sequences, integrons, genomic islands and potential virulence factors in all strains. The structure of the carO porin gene and deduced protein (CarO) were also assessed in CRAb isolates. Results All isolates were found to be multidrug-resistant strains. Antibiotic-resistance genes against six classes of antimicrobial agents were described. The four carbapenem-resistance genes: bla OXA-51 like , bla OXA-23 , bla OXA-24 , and bla OXA-58 genes were detected in 100, 53.3, 13.3, and 6.6% of the isolates, respectively. Additionally, an ISAba1 located upstream of bla OXA-23 and bla ADC-like genes was observed in 53.3 and 66.7% of isolates, respectively. Further, Tn2006 and Tn2008 were found associated to the ISAba1-bla OXA-23 structure. An 8051-bp mobilizable plasmid harbouring the bla OXA-24 gene was isolated in two strains. In addition, 46.7% of isolates were positive for class 1 integrons. Overall, five sequences types (STs), with predominantly ST2, were detected. Several virulence genes were found in the CRAb isolates, among which two genes, epsA and ptk, responsible for the capsule-positive phenotype, were involved in A. baumannii pathogenesis. Conclusions This study revealed the presence of high-level carbapenem resistance in A. baumannii with the first description of OXA-24 and OXA-58 carbapenemases in Madagascar. This highlights the importance of better monitoring and controlling CRAb in Madagascan hospitals to avoid their spread.
Databáze: OpenAIRE
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