Protective effect of Salvia miltiorrhiza Bunge on 5-fluorouracil-induced oral mucositis
| Autor: | Youngjoo Kim, Jinsung Kim, Ha Young Kim, Mun Seog Chang, Do Rim Kim, Ja Young Oh |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Programmed cell death caspase-3 medicine.medical_treatment nuclear factor-κB Inflammation Salvia miltiorrhiza Biology chemotherapy Proinflammatory cytokine Cell Line 03 medical and health sciences 0302 clinical medicine Genetics medicine Mucositis Animals Humans 5-fluorouracil Oral mucosa reactive oxygen species Chemotherapy Stomatitis Mesocricetus Plant Extracts General Medicine Articles medicine.disease 030104 developmental biology medicine.anatomical_structure Apoptosis 030220 oncology & carcinogenesis Cancer research Fluorouracil medicine.symptom Salvia miltiorrhiza Bunge oral mucositis |
| Zdroj: | International Journal of Molecular Medicine |
| ISSN: | 1791-244X |
| Popis: | Oral mucositis is a common side-effect caused by chemotherapy or radiotherapy occurring in the majority of cancer patients and is characterized by inflammation and ulcers in the oral mucosa. In the present study, we examined the protective effects of Salvia miltiorrhiza Bunge (SM) on oral mucositis induced by 5-fluorouracil (5-FU) in human pharyngeal cells and golden Syrian hamsters. We investigated the proliferation and antioxidant abilities of SM using MTT, 2-diphenyl-1-picrylhydrazyl (DPPH) and reactive oxygen species (ROS) assays in vitro. Additionally, TUNEL assay was performed, and the expression levels of nuclear factor-κB (NF-κB), caspase-3 and proinflammatory cytokines were assessed by immunoblotting. The results showed that SM increased the cell proliferation rate in human pharyngeal cells up to 128.97±9.7% compared with this rate in the untreated cells and exerted protective effects on mucosal injury caused by 5-FU treatment. In addition, all concentrations of SM increased DPPH scavenging ability and blocked ROS generation in the treated cells. Taken together, following SM treatment, expression of NF-κB and cleaved caspase-3 were significantly decreased followed by inhibition of cell death. These data suggest that SM could be used for the prevention and treatment of oral mucositis caused by cancer therapies. |
| Databáze: | OpenAIRE |
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