Improved Wound Closure Rates and Mechanical Properties Resembling Native Skin in Murine Diabetic Wounds Treated with a Tropoelastin and Collagen Wound Healing Device
| Autor: | Dominic D. Dominguez, Tatum A. Bardsley, Burt D. Ensley, Aaron J. Tabor, Alison L Ingraldi, Alyssa J. Talbert, Nathan D Cruz, Robert G. Audet, Robert S. Kellar, Robert B. Diller |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
CD31 Pathology medicine.medical_specialty 030209 endocrinology & metabolism Human skin Matrix (biology) Article Extracellular matrix 03 medical and health sciences 0302 clinical medicine Dermis Tropoelastin medicine Diabetic wound Electrospinning integumentary system biology business.industry Wound healing device Pathophysiology Elastin 030104 developmental biology medicine.anatomical_structure biology.protein Wound closure business Wound healing |
| Zdroj: | Journal of diabetes and clinical research |
| DOI: | 10.1101/2020.10.01.322636 |
| Popis: | Chronic wounds in patients suffering from type II diabetes mellitus (DMII) where wounds remain open with a complicated pathophysiology, healing, and recovery process is a public health concern. Normal wound healing plays a critical role in wound closure, restoration of mechanical properties, and the biochemical characteristics of the remodeled tissue. Biological scaffolds provide a tissue substitute to help facilitate wound healing by mimicking the extracellular matrix (ECM) of the dermis. In the current study an electrospun biomimetic scaffold, wound healing device (WHD), containing tropoelastin (TE) and collagen was synthesized to mimic the biochemical and mechanical characteristics of healthy human skin. The WHD was compared to a commercially available porcine small intestinal submucosa (SIS) matrix that has been used in both partial and full-thickness wounds, Oasis® Wound Matrix. Using a diabetic murine model C57BKS.Cg-m+/+Leprdb/J mice (db/db) wound closure rates, histochemistry (CD31 and CD163), qPCR (GAPDH, TNF-α, NOS2, ARG1 and IL10), and mechanical testing of treated wound sites were evaluated. The WHD in a splinted, full thickness, diabetic murine wound healing model demonstrated skin organ regeneration, an enhanced rate of wound closure, decreased tissue inflammation, and a stronger and more durable remodeled tissue that more closely mimics native unwounded skin compared to the control device. |
| Databáze: | OpenAIRE |
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