Principles of stable isotope research – with special reference to protein metabolism
| Autor: | Matthew S. Brook, Daniel J. Wilkinson, Kenneth Smith |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
A-V
Arterial Venous Protein turnover FBR Fractional Breakdown Rate Endocrinology Diabetes and Metabolism FSR Fractional Synthesis Rate Stable isotope tracers Protein metabolism Human metabolism Computational biology Biology chemistry.chemical_compound MPS Muscle Protein Synthesis Ra Rate of Appearance TX341-641 Nutrition and Dietetics Nutrition. Foods and food supply Stable isotope ratio GC-MS Gas Chromatography Mass Spectrometry AP(E) Atom percent (excess) chemistry LC-MS Liquid Chromatography Mass Spectrometry Muscle Original Article Rd Rate of Disappearance Whole body AA Amino Acids |
| Zdroj: | Clinical Nutrition Open Science, Vol 36, Iss, Pp 111-125 (2021) Clinical Nutrition Open Science |
| ISSN: | 2667-2685 |
| DOI: | 10.1016/j.nutos.2021.02.005 |
| Popis: | Summary: The key to understanding the mechanisms regulating disease stems from the ability to accurately quantify the dynamic nature of the metabolism underlying the physiological and pathological changes occurring as a result of the disease. Stable isotope tracer technologies have been at the forefront of this for almost 80 years now, and through a combination of both intense theoretical and technological development over these decades, it is now possible to utilise stable isotope tracers to investigate the complexities of in vivo human metabolism from a whole body perspective, down to the regulation of sub-nanometer cellular components (i.e organelles, nucleotides and individual proteins). This review therefore aims to highlight; 1) the advances made in these stable isotope tracer approaches – with special reference given to their role in understanding the nutritional regulation of protein metabolism, 2) some considerations required for the appropriate application of these stable isotope techniques to study protein metabolism, 3) and finally how new stable isotopes approaches and instrument/technical developments will help to deliver greater clinical insight in the near future. |
| Databáze: | OpenAIRE |
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