Sympathetic innervation regulates macrophage activity in rats with polycystic ovary
| Autor: | Darío Cardozo, Florencia Figueroa, Myriam Forneris, Gisela Mendoza, Liliana Oliveros, Fabian Mohamed |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Sympathetic nervous system Sympathetic Nervous System Endocrinology Diabetes and Metabolism Nitric oxide Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Endocrinology Kisspeptin Internal medicine medicine Animals Androstenedione Cells Cultured Macrophages Ovary Macrophage Activation Polycystic ovary eye diseases Rats Disease Models Animal 030104 developmental biology medicine.anatomical_structure Nerve growth factor chemistry cardiovascular system Female Tumor necrosis factor alpha human activities Polycystic Ovary Syndrome circulatory and respiratory physiology Hormone |
| Zdroj: | Journal of Endocrinology. 238:33-45 |
| ISSN: | 1479-6805 0022-0795 |
| DOI: | 10.1530/joe-17-0736 |
| Popis: | Polycystic ovarian syndrome (PCOS) is a low-grade inflammatory disease characterized by hyperandrogenism and ovarian hyperinnervation. The aim of this work is to investigate whetherin vivobilateral superior ovarian nerve (SON) section in adult rats with estradiol valerate-induced PCOS (PCO rats) affects macrophage spleen cells (MФ) and modifies the steroidogenic ability of their secretions. Culture media of MФ from PCO rats and PCO rats with SON section (PCO-SON rats) were used to stimulatein vitrointact ovaries. Compared with macrophages PCO, macrophages from PCO-SON rats released less tumor necrosis factor-α and nitric oxide, expressed lowerBaxandNfkbmRNA and showed reduced TUNEL staining. Also, in PCO rats, the SON section decreased kisspeptin and nerve growth factor mRNA expressions, without changes inTrkareceptor mRNA levels. Macrophage secretions from PCO-SON rats decreased androstenedione and stimulated progesterone release in PCO ovaries, compared to macrophage secretions from PCO rats. No changes were observed in ovarian estradiol response. These findings emphasize the importance of the SON in spleen MΦ, since its manipulation leads to secondary modifications of immunological and neural mediators, which might influence ovarian steroidogenesis. In PCO ovaries, the reduction of androstenedione and the improvement of progesterone release induced by PCO-SON MΦ secretion, might be beneficial considering the hormonal anomalies characteristic of PCOS. We present functional evidence that modulation of the immune-endocrine function by peripheral sympathetic nervous system might have implications for understanding the pathophysiology of PCOS. |
| Databáze: | OpenAIRE |
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