Interfacial degradation of adhesive composite restorations mediated by oral biofilms and mechanical challenge in an extracted tooth model of secondary caries
| Autor: | Alex Fok, Carola A. Carrera, Yuping Li, Conrado Aparicio, Joel D. Rudney, Ruoquiong Chen |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Molar Dental composite Sucrose Materials science Polyurethanes Composite number Acrylic Resins Dental Plaque Dental Cements Dentistry Dental Caries Composite Resins Article Tooth Fractures 03 medical and health sciences chemistry.chemical_compound Bioreactors 0302 clinical medicine Materials Testing Humans Dental Restoration Permanent Tooth Demineralization General Dentistry business.industry Dental Bonding Biofilm X-Ray Microtomography 030206 dentistry Hydrogen-Ion Concentration Silicon Dioxide Demineralization 030104 developmental biology chemistry Biofilms Dentin Mastication Degradation (geology) Molar Third Zirconium Adhesive business |
| Zdroj: | Journal of Dentistry. 66:62-70 |
| ISSN: | 0300-5712 |
| DOI: | 10.1016/j.jdent.2017.08.009 |
| Popis: | Objective To study the combined effect of simulated occlusal loading and plaque-derived biofilm on the interfacial integrity of dental composite restorations, and to explore whether the effects are modulated by the incorporation of sucrose. Methods MOD-class-II restorations were prepared in third molars. Half of the specimens (n = 27) were subjected to 200,000 cycles of mechanical loading using an artificial oral environment (ART). Then, both groups of specimens (fatigued and non-fatigued) were divided into three subgroups for testing in CDC-reactors under the following conditions: no biofilm (Control), biofilm with no sucrose (BNS) and biofilm pulsed with sucrose (BWS). BNS and BWS reactors were incubated with a multispecies inoculum from a single plaque donor whereas the control reactor was not. The BWS reactor was pulsed with sucrose five times a day. The biofilm challenges were repeated sequentially for 12 weeks. pH was recorded for each run. Specimens were examined for demineralization with micro-CT and load capacity by fast fracture test. Results Demineralization next to the restorations was only detectable in BWS teeth. Fracture loads were significantly reduced by the concomitant presence of biofilm and sucrose, regardless of whether cyclic mechanical loading was applied. Cyclic loading reduced fracture loads under all reactor conditions, but the reduction was not statistically significant. Conclusions Sucrose pulsing was required to induce biofilm-mediated degradation of the adhesive interface. We have presented a comprehensive and clinically relevant model to study the effects of mechanical loading and microbial challenge on the interfacial integrity of dental restorations. |
| Databáze: | OpenAIRE |
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