Genome-wide association study to identify genomic regions and positional candidate genes associated with male fertility in beef cattle
| Autor: | Alexandra M. Livernois, Angela Cánovas, Filippo Miglior, Hannah Sweett, Pablo A. S. Fonseca, Aroa Suárez-Vega |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Agricultural genetics Male Candidate gene Receptors Prolactin media_common.quotation_subject Quantitative Trait Loci lcsh:Medicine Fertility Genome-wide association study Cell Cycle Proteins Quantitative trait locus Beef cattle Biology Article 03 medical and health sciences Gene Frequency Genetic variation Animals lcsh:Science Gene media_common Animal breeding Genetic association study Genetics Multidisciplinary Superoxide Dismutase lcsh:R 0402 animal and dairy science Functional genomics 04 agricultural and veterinary sciences 040201 dairy & animal science 030104 developmental biology Scrotum Sperm Motility lcsh:Q Cattle Chaperonin Containing TCP-1 Genome-Wide Association Study |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Fertility plays a key role in the success of calf production, but there is evidence that reproductive efficiency in beef cattle has decreased during the past half-century worldwide. Therefore, identifying animals with superior fertility could significantly impact cow-calf production efficiency. The objective of this research was to identify candidate regions affecting bull fertility in beef cattle and positional candidate genes annotated within these regions. A GWAS using a weighted single-step genomic BLUP approach was performed on 265 crossbred beef bulls to identify markers associated with scrotal circumference (SC) and sperm motility (SM). Eight windows containing 32 positional candidate genes and five windows containing 28 positional candidate genes explained more than 1% of the genetic variance for SC and SM, respectively. These windows were selected to perform gene annotation, QTL enrichment, and functional analyses. Functional candidate gene prioritization analysis revealed 14 prioritized candidate genes for SC of which MAP3K1 and VIP were previously found to play roles in male fertility. A different set of 14 prioritized genes were identified for SM and five were previously identified as regulators of male fertility (SOD2, TCP1, PACRG, SPEF2, PRLR). Significant enrichment results were identified for fertility and body conformation QTLs within the candidate windows. Gene ontology enrichment analysis including biological processes, molecular functions, and cellular components revealed significant GO terms associated with male fertility. The identification of these regions contributes to a better understanding of fertility associated traits and facilitates the discovery of positional candidate genes for future investigation of causal mutations and their implications. |
| Databáze: | OpenAIRE |
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