Diagnosis of bacteremia in whole-blood samples by use of a commercial universal 16S rRNA gene-based PCR and sequence analysis
| Autor: | Claudia Disqué, Jens Matten, Nele Wellinghausen, Susanne Gebert, Helge Mühl, Juliane Winter, Samir G. Sakka, Anna-Julia Kochem |
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| Rok vydání: | 2009 |
| Předmět: |
Microbiology (medical)
Adult Male Adolescent Bacteremia Biology Polymerase Chain Reaction Sensitivity and Specificity law.invention Microbiology Sepsis law medicine Humans Blood culture Prospective Studies Child Polymerase chain reaction Whole blood Aged Aged 80 and over medicine.diagnostic_test Bacteria Genes rRNA Bacteriology Amplicon Middle Aged medicine.disease 16S ribosomal RNA Molecular biology Real-time polymerase chain reaction Blood Child Preschool Female Reagent Kits Diagnostic Sequence Analysis |
| Zdroj: | Journal of clinical microbiology. 47(9) |
| ISSN: | 1098-660X |
| Popis: | In a prospective, multicenter study of 342 blood samples from 187 patients with systemic inflammatory response syndrome, sepsis, or neutropenic fever, a new commercial PCR test (SepsiTest; Molzym) was evaluated for rapid diagnosis of bacteremia. The test comprises a universal PCR from the 16S rRNA gene, with subsequent identification of bacteria from positive samples by sequence analysis of amplicons. Compared to blood culture (BC), the diagnostic sensitivity and specificity of the PCR were 87.0 and 85.8%, respectively. Considering the 34 BC-positive patients, 28 were also PCR positive in at least one of the samples, resulting in a patient-related sensitivity of 82.4%. The concordance of PCR and BC for both positive and negative samples was (47 + 247)/342, i.e., 86.0%. In total, 31 patients were PCR/sequencing positive and BC negative, in whom the PCR result was judged as possible or probable to true bacteremia in 25. In conclusion, the PCR approach facilitates the detection of bacteremia in blood samples within a few hours. Despite the indispensability of BC diagnostics, the rapid detection of bacteria by SepsiTest appears to be a valuable tool, allowing earlier pathogen-adapted antimicrobial therapy in critically ill patients. |
| Databáze: | OpenAIRE |
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