Novel long noncoding RNAs (lncRNAs) in Myogenesis: A miR-31 overlapping lncRNA transcript controls myoblast differentiation
| Autor: | Tiziana Santini, Chiara Pinnarò, Irene Bozzoni, Valentina Cazzella, Lavinia Bisceglie, Anna Tramontano, Mariangela Morlando, Daniel D'Andrea, Monica Ballarino, Andrea Cipriano, Luigi Grassi |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Duchenne muscular dystrophy
Cellular differentiation Biology Muscle Development Transgenic Transcriptome Striated Myoblasts Mice microRNA medicine RNA Precursors Animals Humans Muscular Dystrophy Molecular Biology Biological Evolution Cell Differentiation Cell Proliferation Gene Expression Profiling Gene Expression Regulation Mice Transgenic MicroRNAs Muscle Smooth Muscle Striated Muscular Dystrophy Duchenne RNA Long Noncoding Cell Biology Regulation of gene expression Myogenesis RNA medicine.disease Duchenne Molecular biology Gene expression profiling Muscle Long Noncoding Smooth |
| Popis: | Transcriptome analysis allowed the identification of new long noncoding RNAs differentially expressed during murine myoblast differentiation. These transcripts were classified on the basis of their expression under proliferating versus differentiated conditions, muscle-restricted activation, and subcellular localization. Several species displayed preferential expression in dystrophic (mdx) versus wild-type muscles, indicating their possible link with regenerative processes. One of the identified transcripts, lnc-31, even if originating from the same nuclear precursor of miR-31, is produced by a pathway mutually exclusive. We show that lnc-31 and its human homologue hsa-lnc-31 are expressed in proliferating myoblasts, where they counteract differentiation. In line with this, both species are more abundant in mdx muscles and in human Duchenne muscular dystrophy (DMD) myoblasts, than in their normal counterparts. Altogether, these data suggest a crucial role for lnc-31 in controlling the differentiation commitment of precursor myoblasts and indicate that its function is maintained in evolution despite the poor sequence conservation with the human counterpart. |
| Databáze: | OpenAIRE |
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