Adriamycin uptake and metabolism in organotypic culture of A 549 human adenocarcinoma cells according to the exposure time

Autor:	Sylvie Chevillard, Philippe Vielh, Jacques Coppey, Gerard Bastian
Rok vydání:	1990
Předmět:	Cancer Research Lung Neoplasms Time Factors Cellular detoxification General Medicine Metabolism Adenocarcinoma Biology In vitro Oncology Biochemistry Doxorubicin Cytoplasm Toxicity Tumor Cells Cultured medicine Humans Efflux Cytotoxicity Chromatography High Pressure Liquid medicine.drug
Zdroj:	Journal of Cancer Research and Clinical Oncology. 116:633-638
ISSN:	1432-1335 0171-5216
Popis:	In organotypic cultures (nodules) of A 549 human lung adenocarcinoma cells, the long-term cytotoxicity of Adriamycin is strongly improved by shortening the exposure time to the drug. In order to gain insight into the mechanisms of Adriamycin toxicity in this system, we have examined the drug uptake, retention and metabolism by fluorescence microscopy and HPLC analysis. A 549 nodules efficiently metabolize Adriamycin, two major metabolites, adriamycinol and an aglycone derivative, as yet chemically unidentified, are formed and efficiently excreted. Kinetic data show that a long exposure to Adriamycin triggers its efflux from both the nucleus and the cytoplasm while stimulating its metabolism. Therefore, a long exposure time to the drug appears to trigger a process of cellular detoxification by favouring its excretion from the cells via increased metabolism.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae5fc58e3ad8ce642856d5bcb03de899 https://doi.org/10.1007/bf01637086 Zobrazit plný text záznamu Full text from SpringerLink