Bioassay-guided fractionation of extracts from Hypericum perforatum in vitro roots treated with carboxymethylchitosans and determination of antifungal activity against human fungal pathogens
| Autor: | Tocci, Noemi, D'Auria, Felicia Diodata, Simonetti, Giovanna, Panella, Simona, Palamara, ANNA TERESA, Aline, Debrassi, Antonio Rodrigues Clovis, Valdir Filho Cechinel, Clovis Antonio Rodrigues, Cechinel, V., Valdir Cechinel Filho, Sciubba, Fabio, Pasqua, Gabriella |
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| Rok vydání: | 2013 |
| Předmět: |
Antifungal
Antifungal Agents dermatophytes Physiology medicine.drug_class Xanthones st john's wort Ethyl acetate Plant Science Fractionation Microbial Sensitivity Tests Plant Roots biyouxanthone d xanthone candida species cryptococcus neoformans candida species Microbiology chemistry.chemical_compound Minimum inhibitory concentration Xanthone Genetics medicine Humans Candida Cryptococcus neoformans Prenylation Chitosan Traditional medicine biology Plant Extracts Hypericum perforatum biology.organism_classification In vitro chemistry Hypericum |
| Zdroj: | Plant physiology and biochemistry : PPB. 70 |
| ISSN: | 1873-2690 |
| Popis: | The aim of this study was to individuate, by bioassay-guided fractionation, promising antifungal fractions and/or constituents from Hypericum perforatum subsp. angustifolium in vitro roots. Treatments with chitosan, O-carboxymethylchitosan (CMC) and its derivatives were used to improve xanthone production in the roots. The bioassay-guided fractionation of CMC-treated roots led to the individuation of an ethyl acetate fraction, containing the highest amount of xanthones (6.8%) and showing the best antifungal activity with minimal inhibitory concentration (MIC) values of 53.82, 14.18, and 36.52 μg/ml, against Candida spp., Cryptococcus neoformans and dermatophytes, respectively. From this fraction the prenylated xanthone, biyouxanthone D has been isolated and represented the 44.59% of all xanthones detected. For the first time in the present paper biyouxanthone D has been found in H. perforatum roots and tested against C. neoformans, dermatophytes, and Candida species. The xanthone showed the greatest antifungal activity against C. neoformans and dermatophytes, with MIC values of 20.16, 22.63 μg/ml. In conclusion, the results obtained in the present study demonstrated that CMC-treated Hpa in vitro root extracts represent a tool for the obtainment of promising candidates for further pharmacological and clinical studies. |
| Databáze: | OpenAIRE |
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