| Popis: |
Callus cell lines of Tecoma sambucifolium obtained from stem explants accumulate up to 8.0% of their dry weight as phenylpropanoid glycosides: the main components were identified as verbascoside, orobanchoside, isoverbascoside and rhodioloside. In fast growing cell lines, selected on medium containing 2,4-dichlorophenoxyacetic acid and kinetin in comparable concentrations, the ratio of the sugar esters was 60:20:3:1, respectively, a product profile similar to that found in the leaves of the intact plant. When cells were selected on medium containing much lower levels of cytokinin compared with auxin, the growth rate was considerably reduced and the product ratio was found to be 25:60:6:4, respectively. None of the cell lines accumulated iridoid glycosides characteristic of the parent plant. |
