Differential tetraspanin genes expression and subcellular localization during mutualistic interactions in Phaseolus vulgaris
| Autor: | Saul Jimenez-Jimenez, Olivia Santana, Manoj-Kumar Arthikala, Luis Cárdenas, Jesús Aguirre, Sandra Yazmin Arzate Salgado, Elisabeth Armada, Kenji Hashimoto, Kazuyuki Kuchitsu, Fernando Lara-Rojas, Carmen Quinto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Root nodule Tetraspanins Cell Membranes Plant Science Pathology and Laboratory Medicine 01 natural sciences Plant Roots Tetraspanin Gene Expression Regulation Plant Mycorrhizae Medicine and Health Sciences Phylogeny Plant Proteins Phaseolus Multidisciplinary biology Plant Anatomy Eukaryota food and beverages Plants Plants Genetically Modified Cell biology Bacterial Pathogens Subcellular Localization Chemistry Experimental Organism Systems Medical Microbiology Physical Sciences embryonic structures Medicine Pathogens Cellular Structures and Organelles Root Nodules Plant Root Hairs Rhizobium Subcellular Fractions Research Article Arabidopsis Thaliana Science Brassica Cell fate determination Root hair Research and Analysis Methods Microbiology Rhizobia Phosphates 03 medical and health sciences Model Organisms Plant and Algal Models Proteus Vulgaris Vesicles Symbiosis Microbial Pathogens fungi Organisms Chemical Compounds Biology and Life Sciences Membrane Proteins Cell Biology Meristem biology.organism_classification Subcellular localization 030104 developmental biology Membrane protein Animal Studies Reactive Oxygen Species Transcriptome 010606 plant biology & botany |
| Zdroj: | PLoS ONE, Vol 14, Iss 8, p e0219765 (2019) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Arbuscular mycorrhizal fungi and rhizobia association with plants are two of the most successful plant-microbe associations that allow the assimilation of P and N by plants, respectively. These mutualistic interactions require a molecular dialogue, i.e., legume roots exude flavonoids or strigolactones which induce the Nod factors or Myc factors synthesis and secretion from the rhizobia or fungi, respectively. These Nod or Myc factors trigger several responses in the plant root, including calcium oscillations, and reactive oxygen species (ROS). Furthermore, superoxide and H2O2 have emerged as key components that regulate the transitions from proliferation to differentiation in the plant meristems. Similar to the root meristem, the nodule meristem accumulates superoxide and H2O2. Tetraspanins are transmembrane proteins that organize into tetraspanin web regions, where they recruit specific proteins into platforms required for signal transduction, membrane fusion, cell trafficking and ROS generation. Plant tetraspanins are scaffolding proteins associated with root radial patterning, biotic and abiotic stress responses, cell fate determination, and hormonal regulation and recently have been reported as a specific marker of exosomes in animal and plant cells and key players at the site of plant fungal infection. In this study, we conducted transcriptional profiling of the tetraspanin family in common bean (Phaseolus vulgaris L. var. Negro Jamapa) to determine the specific expression patterns and subcellular localization of tetraspanins during nodulation or under mycorrhizal association. Our results demonstrate that the tetraspanins are transcriptionally modulated during the mycorrhizal association, but are also expressed in the infection thread and nodule meristem development. Subcellular localization indicates that tetraspanins have a key role in vesicular trafficking, cell division, and root hair polar growth. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |