Nucleotide sequence of the gyrA gene and characterization of ciprofloxacin-resistant mutants of Helicobacter pylori
| ISSN: | 0066-4804 |
|---|---|
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae57a05669b23b25ebdef63a8b097457 https://pubmed.ncbi.nlm.nih.gov/7695290 |
| Rights: | OPEN |
| Přírůstkové číslo: | edsair.doi.dedup.....ae57a05669b23b25ebdef63a8b097457 |
| Autor: | L. E. Bryan, Richard A. Moore, B. Beckthold, Sallene Wong, Amar Kureishi |
| Rok vydání: | 1995 |
| Předmět: |
Molecular Sequence Data
medicine.disease_cause Campylobacter jejuni DNA gyrase Polymerase Chain Reaction Microbiology Ciprofloxacin medicine Pharmacology (medical) Amino Acid Sequence Gene Escherichia coli Peptide sequence Antibacterial agent Pharmacology chemistry.chemical_classification biology Base Sequence Helicobacter pylori Nucleic acid sequence Gene Amplification Drug Resistance Microbial biology.organism_classification bacterial infections and mycoses Amino acid Infectious Diseases DNA Topoisomerases Type II chemistry DNA Gyrase Mutation Research Article |
| Zdroj: | Antimicrobial agents and chemotherapy. 39(1) |
| ISSN: | 0066-4804 |
| Popis: | PCR was used to amplify a 238-bp region from Helicobacter pylori which corresponded to the quinolone resistance-determining region in Escherichia coli. The gyrA gene of H. pylori was cloned and sequenced. An open reading frame of 2,478 nucleotides coded for a polypeptide of 826 amino acids with a calculated molecular mass of 92,508 Da. The amino acid sequence showed an overall 52% identity with other bacterial gyrA genes but was most closely related to the gyrA subunit of Campylobacter jejuni (76.5% identity). Sequencing of the amplification product from ciprofloxacin-resistant mutants of H. pylori revealed four classes of mutations with substitutions at amino acid 87 (Asn-->Lys), amino acid 88 (Ala-->Val), and amino acid 91 (Asp-->Gly, -->Asn, or -->Tyr) and a double substitution at amino acids 91 and 97 (Ala-->Val). Ciprofloxacin-susceptible strains of H. pylori could be transformed to ciprofloxacin resistance by using the amplified fragment from resistant strains as donor DNA. Of the 11 ciprofloxacin-resistant mutants examined, only one did not have an alteration within the quinolone resistance-determining region, suggesting that, in H. pylori, resistance to quinolones is primarily a result of alterations in gyrA. |
| Databáze: | OpenAIRE |
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