Safety and tolerance of lymph node biopsies from chronic HIV-1 volunteers in rural Tanzania

Autor: Manuel Battegay, Claudia Daubenberger, Amina Nyuri, Catherine Mkindi, Theonestina Byakuzana, Ngisi Masawa, Nina Orlova-Fink, Song Ding, Maja Weisser-Rohacek, Thomas Klimkait, Elias Antony Marandu, Giuseppe Pantaleo, Farida Bani
Rok vydání: 2019
Předmět:
Male
Rural Population
Volunteers
0301 basic medicine
lcsh:Medicine
HIV Infections
Tanzania
0302 clinical medicine
030212 general & internal medicine
Chronic
lcsh:QH301-705.5
Lymph node
medicine.diagnostic_test
Surgical wound
General Medicine
Middle Aged
Research Note
medicine.anatomical_structure
Anti-Retroviral Agents
Female
Lymph
medicine.symptom
Adult
medicine.medical_specialty
Lymph node biopsy
Elite controller
Asymptomatic
General Biochemistry
Genetics and Molecular Biology
Young Adult
03 medical and health sciences
Internal medicine
medicine
Humans
lcsh:Science (General)
business.industry
lcsh:R
Reproducibility of Results
Germinal center
KIULARCO cohort
CD4 Lymphocyte Count
Clinical trial
030104 developmental biology
lcsh:Biology (General)
Chronic Disease
HIV-1
Lymph Node Excision
Lymph Nodes
business
Peripheral lymph
lcsh:Q1-390
Zdroj: BMC Research Notes, Vol 12, Iss 1, Pp 1-5 (2019)
BMC Research Notes
ISSN: 1756-0500
Popis: Objective HIV-1 rapidly establishes a persistent infection that can be contained under life-long antiretroviral therapy (ART) but not cured. One major viral reservoir is the peripheral lymph node (LN) follicles. Studying the impact of novel HIV-1 treatment and vaccination approaches on cells residing in germinal centers is essential for rapid progress towards HIV-1 prevention and cure. Results We enrolled 9 asymptomatic adult volunteers with a newly diagnosed HIV-1 infection and CD4 T cell counts ≥ 350/ml. The patients underwent venous blood collection and inguinal lymph node excision surgery in parallel. Mononuclear cells were extracted from blood and tissues simultaneously. Participants were followed up regularly for 2 weeks until complete healing of the surgical wounds. All participants completed the lymph node excision surgery without clinical complications. Among the 9 volunteers, one elite controller was identified. The number of mononuclear cells recovered from lymph nodes ranged from 68 to 206 million and correlated positively with lymph node size. This is the first study to show that lymph node biopsy is a safe procedure and can be undertaken with local experts in rural settings. It provides a foundation for detailed immune response investigations during future clinical trials.
Databáze: OpenAIRE
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