Neopestalotiopsis species presenting wide dye destaining activity: report of a mycelium-associated laccase
| Autor: | Chirlei Glienke, Elisandro Cesar Bruscato, Jaime Paba, Miriam Marzall-Pereira, Rodrigo Aluizio, Daiani Cristina Savi, Vanessa Kava, Lisandra Santos Ferreira-Maba, Lygia Vitoria Galli-Terasawa, Carolina Heyse Niebisch |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Starch
Nitrogen Microorganism Biology Sodium Chloride Wastewater Microbiology 03 medical and health sciences chemistry.chemical_compound Endophytes Coloring Agents DNA Fungal Mycelium Phylogeny 030304 developmental biology Laccase chemistry.chemical_classification 0303 health sciences 030306 microbiology Fungi Biodegradation Hydrogen-Ion Concentration biology.organism_classification Yeast Carbon Enzyme Biodegradation Environmental Biochemistry chemistry Metals Textile Industry Azo Compounds Bacteria Multilocus Sequence Typing |
| Zdroj: | Microbiological research. 228 |
| ISSN: | 1618-0623 |
| Popis: | Wastewaters from textile dyeing industries represent an ecological concern, notably due to the known toxicity of azo dyes to the local microbiome and human health. Although physicochemical approaches are the rule for the treatment of industrial effluents, biological strategies such as enzyme-mediated dye destaining is a promising alternative. Notwithstanding a broad range of microorganisms, including fungi, algae, yeast, and bacteria, display dye-destaining properties, most of the literature has focused in ligninolytic fungi, leaving other classes of organisms somehow ignored. In this study, six endophytic strains isolated from Maytenus ilicifolia were studied for their destaining activity. The phylogenetic and morphological analysis allowed the identification of strain LGMF1504 as Neopestalotiopsis sp. LGMF1504 that decolorized several commercial dyes as the result of a mycelium-associated laccase. The enzyme expression was modulated by carbon and nitrogen content in the culture medium, it was weakly affected by the presence of aromatic compounds and metal ions while some common laccase mediators improved the destaining activity onto dye substrates. The best culture condition observed for laccase activity was a basic culture medium containing 5 g L−1 starch and 15 g L−1 ammonium tartrate. The laccase activity showed low substrate specificity and almost unaltered performance in a wide range of pH values and NaCl concentrations, suggesting the potential of Neopestalotiopsis sp. LGMF1504 for biodegradation approaches. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect