Real time PCR in childhood tuberculosis: a valuable diagnostic tool
| Autor: | Devendra Singh Chauhan, Gajanand Pounikar, Rajeshwar Dayal, Manoj Kumar Singh, Raj Kamal, Haripal Kashyap, Ankur Goyal, Neeraj Kumar Yadav |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Tuberculosis Adolescent India Real-Time Polymerase Chain Reaction Sensitivity and Specificity Mycobacterium tuberculosis Tuberculosis diagnosis Internal medicine RNA Ribosomal 16S medicine Humans Mycobacterium tuberculosis culture Child Childhood tuberculosis biology business.industry Reproducibility of Results medicine.disease biology.organism_classification Body Fluids RNA Bacterial Real-time polymerase chain reaction Research Design Child Preschool Pediatrics Perinatology and Child Health Immunology Female business Meningitis |
| Zdroj: | Indian journal of pediatrics. 82(2) |
| ISSN: | 0973-7693 |
| Popis: | The present study was conducted to detect and quantitate Mycobacterium tuberculosis from various body fluid specimens of cases of tuberculosis by real time PCR technique and compare results with conventional PCR technique and culture. One hundred fifteen children (18 y) with tuberculosis (diagnosed as per IAP guidelines) and 32 disease matched controls from the Department of Pediatrics, S.N. Medical College, Agra, were included in the study. Different body fluids (CSF, gastric aspirate, pleural fluid, ascitic fluid and lymph node aspirate) were subjected to culture, conventional PCR targeting insertion sequence 1S6110 and Real time PCR targeting 16srRNA of Mycobacterium tuberculosis. Real time PCR showed significantly better results than culture in all body fluids (p 0.05). It was superior to conventional PCR in CSF (p 0.05) but showed comparable results in gastric aspirate, pleural fluid, ascitic fluid and lymph node aspirate (p 0.05). Hence, real time PCR is a promising diagnostic tool for childhood tuberculosis, particularly tubercular meningitis. |
| Databáze: | OpenAIRE |
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