Evaluation of a single amino acid substitution at position 79 of human IFN-α2b in interferon-receptor assembly and activity
| Autor: | Samira Talebi, Majid Sadeghizadeh, Fathollah Ahmadpour, Mehdi Zeinoddini, Alireza Saeedinia |
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| Rok vydání: | 2019 |
| Předmět: |
Protein Conformation
CHO Cells Interferon alpha-2 Protein Engineering Antiviral Agents Biochemistry Cricetulus Immune system Cell surface receptor Homologous chromosome Animals Humans Single amino acid Cell Proliferation Receptors Interferon Interferon receptor Chemistry Interferon-alpha General Medicine Protein engineering Recombinant Proteins Cell biology Molecular Docking Simulation Amino Acid Substitution Signal transduction HeLa Cells Biotechnology |
| Zdroj: | Preparative Biochemistry and Biotechnology. 49:735-743 |
| ISSN: | 1532-2297 1082-6068 |
| Popis: | Type I interferons (IFNs) are homologous cytokines that bind to a cell surface receptor and establish signaling pathways that motivate immune responses. The purpose of the current study is to assess the activity of a novel-engineered IFN-α2b. The crystallographic structure of IFN-α2b and its receptors was acquired from Protein Data Bank. Various amino acid substitutions were designed based on structural properties and other biological characteristics of residues to find the most effective amino acid on IFN affinity to advanced activities. The IFN-α2b mutants and receptors have been modeled and the interactions between two proteins have been studied as |
| Databáze: | OpenAIRE |
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