Hypoxia-cultured mouse mesenchymal stromal cells from bone marrow and compact bone display different phenotypic traits
| Autor: | Juliana de Matos Maçonetto, Fernanda Ursoli Ferreira, Josiane Serrano Borges, Simone Kashima, Péricles Natan Mendes da Costa, Nayara Patrícia Morotti, Rafaela Rossetti, Julianne V. de Carvalho, Dimas Tadeu Covas, Lucas Eduardo Botelho de Souza, Felipe Augusto Rós |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Cell Bone Marrow Cells Biology Immunophenotyping Mice 03 medical and health sciences 0302 clinical medicine Osteogenesis Cortical Bone medicine Animals Clonogenic assay Cells Cultured Cell Proliferation Endosteum Adipogenesis Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Cell Biology Hypoxia (medical) Cell Hypoxia In vitro Cell biology Mice Inbred C57BL Phenotype 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Bone marrow medicine.symptom |
| Zdroj: | Experimental Cell Research. 399:112434 |
| ISSN: | 0014-4827 |
| Popis: | It has been suggested that the bone marrow microenvironment harbors two distinct populations of mesenchymal stromal cells (MSC), one with a perivascular location and other present in the endosteum. A better understanding of the biology of these MSC subsets has been pursued in order to refine its clinical application. However, most comparative characterizations of mouse MSC have been performed in normoxia. This can result in misleading interpretations since mouse MSC subsets with low/defective p53 activity are known to be selected during culture in normoxia. Here, we report a comprehensive in vitro characterization of mouse MSC isolated from bone marrow (BM-MSC) and compact bone (CB-MSC) expanded and assayed under hypoxia for their morphology, clonogenic efficiency and differentiation capacity. We found that, under hypoxia, compact bone is richer in absolute numbers of MSC and isolation of MSC from compact bone is associated with a reduced risk of hematopoietic cell carryover. In addition, CB-MSC have higher in vitro osteogenic capacity than BM-MSC, while adipogenic differentiation potential is similar. These findings reinforce the hypothesis of the existence of MSC in bone marrow and compact bone representing functionally distinct cell populations and highlight the compact bone as an efficient source of murine MSC under physiological oxygen concentrations. |
| Databáze: | OpenAIRE |
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