Distinct peripheral blood RNA responses to Salmonella in pigs differing in Salmonella shedding levels: intersection of IFNG, TLR and miRNA pathways

Autor: Shawn M. D. Bearson, Susan M. Knetter, Jolita J. Uthe, Amanda E. Ramer-Tait, Christopher K. Tuggle, Cumhur Yusuf Demirkale, Ting Hua Huang, Michael J. Wannemuehler, Dan Nettleton, Curtis Christian
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Salmonella typhimurium
Salmonella
Sus scrofa
Gene Expression
medicine.disease_cause
Polymerase Chain Reaction
Gene expression
Molecular Cell Biology
CEBPB
Genetics of the Immune System
Pathology
Cluster Analysis
Gene Regulatory Networks
Immune Response
Animal Management
Oligonucleotide Array Sequence Analysis
Regulation of gene expression
Bacterial Shedding
education.field_of_study
Multidisciplinary
Systems Biology
Toll-Like Receptors
Agriculture
Genomics
Functional Genomics
Salmonella enterica
Area Under Curve
Medicine
Research Article
Signal Transduction
Clinical Pathology
Science
Population
Immunology
Immunopathology
Biology
Molecular Genetics
Immunomodulation
Interferon-gamma
Diagnostic Medicine
medicine
Genetics
Animals
Gene Regulation
RNA
Messenger
Gene Networks
education
Salmonella Infections
Animal
Gene Expression Profiling
Computational Biology
Reproducibility of Results
Molecular Sequence Annotation
biology.organism_classification
Gene expression profiling
MicroRNAs
Gene Expression Regulation
Immune System
Clinical Immunology
Veterinary Science
Gene Function
Genome Expression Analysis
Animal Genetics
Zdroj: PLoS ONE, Vol 6, Iss 12, p e28768 (2011)
PLoS ONE
ISSN: 1932-6203
Popis: Transcriptomic analysis of the response to bacterial pathogens has been reported for several species, yet few studies have investigated the transcriptional differences in whole blood in subjects that differ in their disease response phenotypes. Salmonella species infect many vertebrate species, and pigs colonized with Salmonella enterica serovar Typhimurium (ST) are usually asymptomatic, making detection of these Salmonella-carrier pigs difficult. The variable fecal shedding of Salmonella is an important cause of foodborne illness and zoonotic disease. To investigate gene pathways and biomarkers associated with the variance in Salmonella shedding following experimental inoculation, we initiated the first analysis of the whole blood transcriptional response induced by Salmonella. A population of pigs (n = 40) was inoculated with ST and peripheral blood and fecal Salmonella counts were collected between 2 and 20 days post-inoculation (dpi). Two groups of pigs with either low shedding (LS) or persistent shedding (PS) phenotypes were identified. Global transcriptional changes in response to ST inoculation were identified by Affymetrix Genechip® analysis of peripheral blood RNA at day 0 and 2 dpi. ST inoculation triggered substantial gene expression changes in the pigs and there was differential expression of many genes between LS and PS pigs. Analysis of the differential profiles of gene expression within and between PS and LS phenotypic classes identified distinct regulatory pathways mediated by IFN-γ, TNF, NF-κB, or one of several miRNAs. We confirmed the activation of two regulatory factors, SPI1 and CEBPB, and demonstrated that expression of miR-155 was decreased specifically in the PS animals. These data provide insight into specific pathways associated with extremes in Salmonella fecal shedding that can be targeted for further exploration on why some animals develop a carrier state. This knowledge can also be used to develop rational manipulations of genetics, pharmaceuticals, nutrition or husbandry methods to decrease Salmonella colonization, shedding and spread.
Databáze: OpenAIRE
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