External GSSG enhances intracellular glutathione level in isolated cardiac myocytes
| Autor: | Isabella Vaona, Rolando Budini, Carlo Guarnieri, A. Fraticelli, Carlo Ventura |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
inorganic chemicals
medicine.medical_specialty Cell Glutathione reductase Biophysics Biochemistry Acetylcysteine chemistry.chemical_compound fluids and secretions Internal medicine medicine Animals Myocyte Buthionine sulfoximine Cysteine Molecular Biology Incubation Myocardium Maleates Heart Rats Inbred Strains Cell Biology Glutathione Carmustine Rats Kinetics Glucose medicine.anatomical_structure Endocrinology chemistry Oxidation-Reduction medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 147:658-665 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(87)90981-8 |
| Popis: | The addition of external GSSG at concentrations in the range 50–500 μM produces in isolated adult rat heart myocytes an increase of GSH level and only a slight increase of GSSG level. On the contrary, external GSH at the above same indicated concentrations did not change the cell glutathione pool. The pretreatment of the cells with diethylmaleate depleted the myocytes of glutathione and enhanced the GSSG-induced replenishment effect on GSH level. On the contrary, the addition of GSH did not increase the concentration of cell glutathione. The level of cell GSH in diethylmaleate-treated myocytes was not increased after 30 min of incubation with cysteine, or acetylcysteine. The GSSG induced-stimulation on GSH level was not inhibited by buthionine sulfoximine, an inhibitor of glutathione synthesis. On the contrary, this stimulatory effect was inhibited by N, N-bis(2-chloroethyl)-N-nitrosourea, an inhibitor of glutathione reductase, or partially, by the remotion of glucose from the incubation medium. These results support the idea that the isolated adult rat heart myocytes are able to utilize external GSSG in order to increase the intracellular glutathione pool, probably through the reduction of the imported GSSG to GSH. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|