Attenuation of Hyperoxic Lung Injury in Newborn Thioredoxin-1-Overexpressing Mice through the Suppression of Proinflammatory Cytokine mRNA Expression
| Autor: | Junichi Ozawa, Nobuhiko Nagano, Takaaki Watanabe, Fuyu Miyake, Shun Matsumura, Kosuke Tanaka, Fumihiko Namba, Kohei Osada, Masanori Tamura, Kikumi Matsuoka |
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| Rok vydání: | 2020 |
| Předmět: |
Chemokine
animal structures Medicine (miscellaneous) Lung injury hyperoxic lung injury General Biochemistry Genetics and Molecular Biology Article Proinflammatory cytokine antioxidant effects Andrology 03 medical and health sciences 0302 clinical medicine newborn mice 030225 pediatrics Gene expression medicine 030212 general & internal medicine lcsh:QH301-705.5 Hyperoxia biology thioredoxin-1 Chemistry Monocyte respiratory system respiratory tract diseases medicine.anatomical_structure lcsh:Biology (General) biology.protein medicine.symptom Elastin Immunostaining proinflammatory cytokine gene expression |
| Zdroj: | Biomedicines Biomedicines, Vol 8, Iss 3, p 66 (2020) Volume 8 Issue 3 |
| ISSN: | 2227-9059 |
| Popis: | The role of thioredoxin-1 (TRX), a small redox-active protein with antioxidant effects, during hyperoxic lung injury in newborns remains undetermined. We investigated TRX impact on hyperoxic lung injury in newborn TRX transgenic (TRX-Tg) and wildtype (WT) mice exposed to 21% or 95% O2 for four days, after which some mice were allowed to recover in room air for up to 14 days. Lung morphology was assessed by hematoxylin/eosin and elastin staining, as well as immunostaining for macrophages. The gene expression levels of proinflammatory cytokines were evaluated using quantitative real-time polymerase chain reaction. During recovery from hyperoxia, TRX-Tg mice exhibited an improved mean linear intercept length and increased number of secondary septa in lungs compared with the WT mice. Neonatal hyperoxia enhanced the mRNA expression levels of proinflammatory cytokines in the lungs of both TRX-Tg and WT mice. However, interleukin-6, monocyte chemoattractant protein-1, and chemokine (C-X-C motif) ligand 2 mRNA expression levels were reduced in the lungs of TRX-Tg mice compared with the WT mice during recovery from hyperoxia. Furthermore, TRX-Tg mice exhibited reduced macrophage infiltration in lungs during recovery. These results suggest that in newborn mice TRX ameliorates hyperoxic lung injury during recovery likely through the suppression of proinflammatory cytokines. |
| Databáze: | OpenAIRE |
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