Jingshu Keli attenuates cervical spinal nerve ligation-induced allodynia in rats through inhibition of spinal microglia and Stat3 activation
| Autor: | Xinluan Wang, Jiang Yue, Shihui Chen, Nianye Zheng, Idy H. T. Ho, Jiankun Xu, Ri Zhang, Hao Yao, Ling Qin, Xiaodong Liu, Jiali Wang |
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| Rok vydání: | 2018 |
| Předmět: |
Male
STAT3 Transcription Factor 0301 basic medicine Context (language use) Pharmacology Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine In vivo Animals Medicine Orthopedics and Sports Medicine Radiculopathy STAT3 Microglia biology business.industry Spinal cord medicine.disease Rats Spinal Nerves 030104 developmental biology Allodynia medicine.anatomical_structure Spinal Cord Hyperalgesia Radicular pain Spinal nerve biology.protein Surgery Neurology (clinical) medicine.symptom business 030217 neurology & neurosurgery Drugs Chinese Herbal |
| Zdroj: | The Spine Journal. 18:2112-2118 |
| ISSN: | 1529-9430 |
| DOI: | 10.1016/j.spinee.2018.06.354 |
| Popis: | BACKGROUND CONTEXT Cervical radicular pain resulting from mechanical compression of a spinal nerve secondary to spinal degenerative alternations negatively impacts patients’ quality of life. Jingshu Keli (JSKL), a traditional Chinese medicine formula with multiple active compounds, has been prescribed for pain management in patients with cervical radiculopathy for decades. Two major components of JSKL, ferulic acid and cinnamaldehyde, were identified to have anti-inflammation effect via inhibiting activation of Stat3. PURPOSE To investigate the efficacy of JSKL by investigating its mechanism in attenuating cervical radiculopathy-induced mechanical allodynia via modulation activation of spinal microglia and phosphorylation of signal transducer and activator of transcription 3 (Stat3). STUDY DESIGN An in vivo animal experiment. METHODS Cervical radiculopathy of rats was established by C7 spinal nerve ligation (SNL) with 6-0 silk suture. The effect of postoperational daily gavage of JSKL on mechanical allodynia of rats was tested on day 3, 7, and 14 after surgery. Furthermore, spinal glial cells activation and phosphorylation of Stat3 (p-Stat3) were tested with immunofluorescence imaging and Western blot. RESULT The JSKL significantly inhibited SNL-induced allodynia as well as microglia activation in the spinal cord on day 7 and 14 after surgery. Moreover, expression of p-Stat3 was decreased in rats with SNL and JSKL treatment in comparison with rats with SNL and vehicle treatment. CONCLUSIONS The JSKL attenuated SNL-induced mechanical allodynia in rats. This analgesic effect might be explained by the suppression of activations of spinal microglia as well as p-Stat3. Our study provides experimental evidence for JSKL as an alternative approach to manage refractory pain in patients with cervical radiculopathy. |
| Databáze: | OpenAIRE |
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