Prokaryotic Diversity of Hot Springs in Balikesir and Kutahya, Turkey
Autor: | Emine Çelikoğlu, Meral Yılmaz Cankılıç |
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Přispěvatelé: | Anadolu Üniversitesi, Fen Fakültesi, Biyoloji Bölümü |
Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0106 biological sciences
0301 basic medicine Cloning media_common.quotation_subject Thermophile Zoology 16S Rrna Biology 16S ribosomal RNA 01 natural sciences Ardra 010601 ecology 03 medical and health sciences 030104 developmental biology Fish Fish Thermophilic Bacteria Agronomy and Crop Science Ecology Evolution Behavior and Systematics Diversity (politics) media_common |
Popis: | WOS: 000387850600026 The prokaryotic diversity of the hot springs Sindirgi, Gure, and Havran in the Balikesir Province and Eynal in the Kutahya Province, both located in the western part of central Anatolia, was analyzed and compared using cultivation and cultivation-independent methods, including Fluorescence In Situ Hybridization (FISH) and cloning of PCR-amplified fragments of 16S rRNA genes. A total of 66 isolates were obtained from the cultures. They were identified to the species or genus level as Geobacillus, Bacillus, Brevibacillus, Aneurinibacillus, Anoxybacillus, and Aeribacillus. As a result of the FISH study, only bacteria domain signals were received, but arkea domain signals were not. A total of 265 clones from the 16S rRNA gene library were analyzed with Amplified Ribosomal DNA Restriction Analysis. These bacterial clones were identified as the genera Anoxybacillus, Meiothermus, Uncultured bacterium, Aneurinibacillus, Brevibacillus, Bacillus, and Geobacillus. This research highlights the prokaryotic diversity of hot springs in the Balikesir and Kutahya Provinces. Anadolu University Research Project Department [1403F100] The authors would like to thank Anadolu University Research Project Department, Project 1403F100. |
Databáze: | OpenAIRE |
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